硫酸化制备圆苞车前子低黏多糖及其结构表征和抗氧化活性研究  

Preparation of low viscosity polysaccharide from Plantago ovata Forsk by sulfation method and its structural characterization and antioxidant activities

作　　者：曹磊 刘伟 王俊龙 纳森巴特 巴哈尔古丽·别克吐尔逊 松布尔 冯亚萍 热合巴提·努尔夏提 CAO Lei;LIU Wei;WANG Jun-long;NASEN Bate;BIEKETUERXUN Bahaerguli;SONG Bu-er;FENG Ya-ping;NUERXIATI Rehebati(Key Laboratory of Natural Product Chemistry and Application,School of Chemistry and Chemical Engineering,Yili Normal University,Yining 835000,China)

机构地区：[1]伊犁师范大学化学化工学院、新疆维吾尔自治区教育厅天然产物化学与应用重点实验室,伊宁835000

出　　处：《天然产物研究与开发》2024年第1期26-36,共11页Natural Product Research and Development

基　　金：“新疆维吾尔自治区高校科研计划项目”(XJEDU2022P093);“新疆维吾尔自治区科研创新项目”(XJ2023G263);“天池英才”引进计划(2023TCYCQNBS03);2022年伊犁师范大学高层次人才科研启动金(2022RCYJ04)。

摘　　要：圆苞车前子(Plantago ovata Forsk)具有润肠通便,辅助消化等功效,但因高黏特性无法对其多糖成分展开系统研究。因此,本研究以圆苞车前子为研究对象,采用氯磺酸-吡啶法制备低黏性圆苞车前子硫酸化多糖(POFP-S),POFP-S经DEAE-650M和Superdex 200凝胶分离纯化获得一种中性和一种酸性均一多糖(POFP-S1、POFP-S2),其中POFP-S1主要由鼠李糖、阿拉伯糖、木糖和葡萄糖组成,分子量为3556 kDa,硫酸基取代度为0.364;POFP-S2由阿拉伯糖和木糖组成,分子量为833.7 kDa,硫酸基取代度为1.080;红外光谱(FT-IR)中典型的硫酸基吸收峰证实了硫酸化修饰的成功。X射线衍射(XRD)及刚果红实验结果显示,POFP-S2是具三螺旋结构的,晶体与非晶体结构共存的特殊多糖,也表明硫酸化修饰未影响圆苞车前子多糖的立体结构。采用DPPH、ABTS及OH等自由基清除实验,考察了硫酸化多糖的体外抗氧化活性,发现POFP-S1和POFP-S2具有显著的抗氧化活性。研究结果将为进一步研究圆苞车前子多糖的结构与活性提供文献依据,以及开发具有潜力的药食同源产品提供依据,并为高黏性多糖的研究提供理论参考。Plantago ovata Forsk has laxative and digestive effects,but due to its high viscosity,it is difficult to conduct systematic research on its polysaccharide components.Therefore,in this study,the low viscosity sulfated polysaccharide of P.ovata(POFP-S)was prepared by chlorosulfonic acid-pyridine method.,POFP-S was further separated and purified by DEAE-650M and Superdex 200 column to obtained a neutral and an acidic homogeneous polysaccharide(POFP-S1,POFP-S2),of which the POFP-S1 was mainly composed of rhamnose,arabinose,xylose,and glucose,with a molecular weight of 3556 kDa and a degree of sulfate substitution of 0.364,while POFP-S2 was composed of arabinose and xylose,with a molecular weight of 833.7 kDa and a degree of sulfate substitution of 1.080.The characteristic absorption peaks in infrared spectroscopy(FT-IR)confirm the success of sulfation modification.The results of X-ray diffraction(XRD)and Congo red test show that both POFP-S1 and POFP-S2 have a triple helix structure,and with a crystalline and amorphous structure.This also indicates that sulfation modification does not affect the three-dimensional structure of the polysaccharides.The in vitro antioxidant activities of the sulfated polysaccharides were studied using DPPH,ABTS and OH free radical scavenging assays,and it was found that both POFP-S1 and POFP-S2 exhibited antioxidant activity.The results will provide a literature basis for further research on the structure and activity of polysaccharides from P.ovata,as well as the development of potential medicinal and food products and provide theoretical references for the study of high viscosity polysaccharides.

关 键 词：圆苞车前子多糖 硫酸化 抗氧化活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]