地卷柏的化学成分及其α-葡萄糖苷酶抑制活性研究  

Chemical constituents from Selaginella prostrata H.S.Kung and theirα-glucosidase inhibitory activities

作　　者：姜红宇 吕敬崑 邵金华 陈小明 JIANG Hong-yu;LYU Jing-kun;SHAO Jin-hua;CHEN Xiao-ming(School of Chemistry and Bioengineering,Hunan University of Science and Engineering;Hunan Provincial Engineering Research Center for Ginkgo Folium,Hunan University of Science and Engineering;Hunan Hengwei Pharmaceutical Co.,Ltd.,Yongzhou 425100,China)

机构地区：[1]湖南科技学院化学与生物工程学院 [2]湖南省银杏工程技术研究中心 [3]湖南恒伟药业股份有限公司,永州425100

出　　处：《天然产物研究与开发》2024年第1期37-43,共7页Natural Product Research and Development

基　　金：湖南省自然科学基金(2020JJ9026)。

摘　　要：研究地卷柏(Selaginella prostrata H.S.Kung)的化学成分及其α-葡萄糖苷酶抑制活性,综合运用硅胶、Sephadex LH-20和半制备HPLC等多种色谱分离技术,从地卷柏全草的95%乙醇提取物中分离得到10个化合物,通过现代波谱学方法鉴定其结构为seprostrataoid A(1)、(3 S,5 R,8 S)-5,8-epoxy-6-megastigmadien-3,9-diol(2)、(-)-黑麦草内酯(3)、(3 S,5 R,6 R,7 E)-3,5,6-三羟基-7-巨豆烯-9-酮(4)、(7 R,8 S)-ceplignan(5)、(2 R,3 S)-二氢-2-(3′,5′-二甲氧基-4′-羟基苯基)-3-羟甲基-7-甲氧基-5-乙酰基苯骈呋喃(6)、5,5′-二甲氧基木香酚A(7)、穗花衫双黄酮(8)、异柳杉双黄酮(9)、扁柏双黄酮(10)。其中,化合物1为新的倍半萜类化合物,化合物2~10为首次从地卷柏中分离得到。α-葡萄糖苷酶抑制活性测试结果显示,化合物7~10具有较好的α-葡萄糖苷酶抑制活性,IC 50值分别为72.14±2.67、42.68±1.54、61.26±4.32、55.43±2.17μmol/L。This paper aims to study the chemical constituents of Selaginella prostrata H.S.Kung and theirα-glucosidase inhibitory activity.A new sesquiterpene,namely seprostrataoid A(1),together with nine known compounds,namely(3 S,5 R,8 S)-5,8-epoxy-6-megastigmadien-3,9-diol(2),(-)-loliolide(3),(3 S,5 R,6 R,7 E)-3,5,6-trihydroxy-7-megastigmen-9-one(4),(7 R,8 S)-ceplignan(5),(2 R,3 S)-dihydro-2-(3′,5′-dimethoxy-4′-hydroxyphenyl)-7-methoxy-5-acetyl-benzofuran(6),5,5′-dimethoxylclemaphenol A(7),amentoflavone(8),isocryptomerin(9),hinokiflavone(10),were isolated and purified from the 95%ethanol extract of S.prostrata by comprehensive chromatography methods of silica gel column,Sephadex LH-20 gel column and semi-preparative HPLC.All compounds are obtained from S.prostrata for the first time.In vitro activity results showed that compounds 7-10 had significantα-glucosidase inhibitory activity with IC 50 values of 72.14±2.67,42.68±1.54,61.26±4.32 and 55.43±2.17μmol/L,respectively.

关 键 词：地卷柏 化学成分 倍半萜 双黄酮 Α-葡萄糖苷酶 

分 类 号：R284.1[医药卫生—中药学]