乌鳢鱼皮中胶原蛋白的提取工艺及特性研究  

Isolation and characterization of collagens from skin of snakehead fish

作　　者：温庆仕 陈柏杨 王松涛 童钰琴 温福丽 周精卫 陈勇 WEN Qingshi;CHEN Baiyang;WANG Songtao;TONG Yuqin;WEN Fuli;ZHOU Jingwei;CHEN Yong(Jiangsu Jicui Industrial Biotechnology Research Institute Co.Ltd.,Nanjing 210032,China;Luzhou Pinchuang Technology Company Limited,Luzhou 646000,China;National Engineering Research Center of Solid-State Brewing,Luzhou 646000,China;Nanjing Hi Tech Biological Technology Research Institute Co.Ltd.,Nanjing 210000,China;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China)

机构地区：[1]江苏集萃工业生物技术研究所有限公司,江苏南京210032 [2]泸州品创科技有限公司,四川泸州646000 [3]国家固态酿造工程技术研究中心,四川泸州646000 [4]南京高新工大生物技术研究院有限公司,江苏南京210000 [5]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《食品与发酵工业》2024年第1期219-224,共6页Food and Fermentation Industries

基　　金：第十五批江苏省“六大人才高峰”高层次人才项目(JNHB-154);江苏省重点研发计划项目(BE2019001)。

摘　　要：该研究利用醋酸和胃蛋白酶结合从乌鳢鱼皮中提取胶原蛋白。采用单因素试验和正交试验考察了醋酸浓度、料液比、酶用量、提取时间等条件对胶原蛋白提取率的影响。结果表明,当醋酸的浓度为0.5 mol/L,胃蛋白酶的添加量为鱼皮质量的1%,提取时间48 h,料液比1∶70,胶原蛋白提取率达到51.2%。通过氨基酸组成分析、圆二色谱、聚丙烯酰胺凝胶电泳等方法对胶原蛋白的结构进行测试,发现该法提取的胶原蛋白具有典型Ⅰ型胶原蛋白特征,其三螺旋结构保持完整,分子质量约为340 kDa。成品中蛋白质含量>95%,羟脯氨酸的含量>5%,灰分<1%(均为质量分数),可用于医用材料、化妆品、功能食品等领域。In this research,collagen was extracted from the skin of snakehead fish by the combination of acetic acid and pepsin.The effects of acetic acid concentration,solid-liquid ratio,enzyme dosage,and extraction time on the extraction rate of collagen were investigated by single factor experiment and orthogonal test.Results showed that when the concentration of acetic acid was 0.5 mol/L,the addition of pepsin was 1%of the weight of fish skin,the extraction time was 48 h,and the solid-liquid ratio was 1∶70,the extraction rate of collagen reached 51.2%.The structure of collagen was tested by amino acid composition analysis,circular dichroism,and polyacrylamide gel electrophoresis.It was found that the collagen extracted by this method had typical type I collagen characteristics,its 3-helix structure remained intact,and its molecular weight was about 340 kDa.The protein content of the final goods was more than 95%,the hydroxyproline content was more than 5%,and the ash content was less than 1%,which could be used in medical materials,cosmetics,functional foods and other fields.

关 键 词：乌鳢鱼皮 醋酸 胶原蛋白 胃蛋白酶 提取 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]