Periostin表达在乳恒牙替换中的临床意义  

Clinical significance of Periostin expression in the replacement of primary permanent teeth

作　　者：张勇[1] 张洪兰 秦晗 ZHANG Yong;ZHANG Honglan;QIN Han(Department of Stomatology,Lianyungang Clinical Medical College Nanjing Medical University,Lianyungang 222000,Jiangsu,China;Department of Pathology,Lianyungang Clinical Medical College Nanjing Medical University,Lianyungang 222000,Jiangsu,China)

机构地区：[1]南京医科大学连云港临床医学院口腔科,江苏连云港222000 [2]南京医科大学连云港临床医学院病理科,江苏连云港222000

出　　处：《中国现代医生》2024年第2期1-4,45,共5页China Modern Doctor

基　　金：国家自然科学基金项目(81500893)。

摘　　要：目的 探讨Periostin在替牙期对骨改建和自噬的影响,研究Periostin在乳恒牙替换过程中调节骨代谢平衡的作用机制。方法 收集笔者所在医院因乳牙根尖周病及多生牙拔除的牙根周围粘连牙槽骨,体外培养成骨细胞,分为实验组和对照组各15例,实验组培养基中加Periostin,原位杂交法检测标本中Periostin、骨代谢基因、自噬基因的m RNA水平。蛋白质印记法检测成骨细胞中骨代谢蛋白,自噬蛋白表达。结果 原位杂交结果显示,实验组Periostin表达低于对照组(P<0.05),核因子κB受体活化因子配体(receptor activator of nuclear factor-κb ligand,RANKL)、LC3、BECN1表达及RANKL/骨保护素(osteoprotegerin,OPG)值高于对照组(均P<0.05),OPG表达差异无统计学意义(P>0.05);蛋白质印迹法显示实验组RANKL、RANKL/OPG、LC3Ⅱ、Beclin-1、LC3Ⅱ/LC3Ⅰ与对照组比较水平低(均P<0.05),OPG、LC3Ⅰ较对照组差异无统计学意义(均P>0.05)。结论 Periostin通过降低RANKL表达及改善过强自噬状态,从而减少炎症所致的骨吸收。Objective To investigate the effect of periostin on bone remodeling and autophagy during the period of tooth replacement and to investigate the mechanism of action of periostin in regulating the balance of bone metabolism during the replacement of primary and permanent teeth.Methods Fifteen cases each of experimental and control groups were collected from the periapical periodontal disease and multiple teeth extracted for periapical adhesions in the author’s hospital,the mRNA levels of periostin,bone metabolism genes,and autophagy genes were detected in the specimens by in situ hybridization.Osteoblasts were cultured in vitro,periostin was added to the culture medium of the experimental group,and the expression of bone metabolism proteins[receptor activator of nuclear factor-κb ligand(RANKL),osteoprotegerin(OPG)]and autophagy proteins(LC3,Beclin-1)in osteoblasts was detected by Western blot.Results In situ hybridization results showed that periostin expression in the experimental group was lower than that in the control group(P<0.05),RANKL,LC3,BECN1 expression and RANKL/OPG values were higher than those in the control group(all P<0.05),and no significant differences were seen in OPG expression(P>0.05);protein blotting showed that RANKL,RANKL/OPG,LC3Ⅱ,Beclin-1,LC3Ⅱ/LC3Ⅰwere lower in the experimental group than in the control group(all P<0.05),and no significant differences were seen in OPG and LC3Ⅰthan in the control group(P>0.05).Conclusion Periostin reduces inflammation-induced bone resorption by reducing RANKL expression and improving hyperautophagic state.

关 键 词：骨膜蛋白 骨改建 乳恒牙替换 自噬 

分 类 号：R788[医药卫生—口腔医学]