喀斯特洞穴细菌群落的生境特异性及其潜在功能:以广西桂林盘龙洞为例  

Niche Specificity and Potential Functions of Microbial Communities in Karst Caves as Exampled by Panlong Cave in Guilin City,Guangxi

作　　者：曾智霖 程晓钰 王红梅[1,2] 曹静 杨梓琪 刘晓燕 王昳衡[1,2] 李璐 苏春田 黄奇波[3] Zeng Zhilin;Cheng Xiaoyu;Wang Hongmei;Cao Jing;Yang Ziqi;Liu Xiaoyan;WangYiheng;Li Lu;Su Chuntian;Huang Qibo(School of Environmental Studies,China University of Geosciences,Wuhan 430078,China;State Key Laboratory of Geobiology and Environmental Geology,China University of Geosciences,Wuhan 430078,China;Institute of Karst Geology,China Geological Survey,Guilin 541000,China)

机构地区：[1]中国地质大学环境学院,湖北武汉430078 [2]中国地质大学生物地质与环境地质国家重点实验室,湖北武汉430078 [3]中国地质调查局岩溶地质研究所,广西桂林541000

出　　处：《地球科学》2023年第12期4711-4726,共16页Earth Science

基　　金：国家自然科学基金重点项目(No.91951208);广西自然科学基金项目(No.2020GXNSFAA297025);中国地质调查局地质调查项目(No.DD20190343)联合资助。

摘　　要：开展岩溶洞穴不同生境微生物与环境因子间的研究,对阐明深部生物圈微生物的多样性、潜在功能及环境驱动机制具有重要意义.以广西桂林盘龙洞为例,通过对细菌16S rRNA高通量测序系统研究了洞穴7种小生境细菌群落的空间分布特征及其与环境因子之间的关系.研究发现温度是驱动盘龙洞细菌群落组成的重要因素,微生物群落组成及潜在生态功能均具有生境特异性,不同生境具有独有的细菌指示类群.滴水、干燥石笋表面生物膜、湿润石笋表面生物膜3个生境与洞穴氮循环密切相关,风化结皮、沉积物、岩壁3个生境与洞穴微生物固定二氧化碳关系紧密.此外,洞穴细菌通过密切的代谢交换形成协作的正相关关系,暗示着微生物在洞穴这一极端环境中的生存策略.Karst caves serve as the natural laboratories to study subsurface biosphere,which provide ideal places to investigate microbial diversity and their interactions between microbes and environments.Guilin is one of the typical areas with developed karst landscape.Deep investigation of cave microbes in different niches and their response to environmental variables will provide valuable information on subsurface biosphere in terms of microbial diversity,function and interaction with environments.To this end,Samples were collected from overlying soils,sediments,cave wall,weathered crusted on cave walls,microbial biofilm on stalagmite surface and dripping water in the Panlong Cave in Guiling City to study microbial communities and their correlation with environmental variables via high throughput sequencing of 16S rRNA.Results show that bacterial communities show high specificity to niches and were dominated by Actinomycete and Proteobacteria.Each niche has its own specific indicator group.For example,Paemibacillus was the indicator for microbial biofilm on the dry surface of stalagmite,whilst Acidobacteriaceae and Pseudomonas were the indicator groups in microbial biofilm on the wet surface of stalagmite.Pseudomonadales and Branhamella were the indicator groups in dripping water,whereas indicator groups in overlying soils included Mycobacterium and Nocardioides.Bacillus and Gp-7 were indicators in sediments,whilst Chromatiales,Soilrubrobacteraceae and Rubrobcter were indicators in cave wall and Methylobacterium in weathered crust.Temperature was demonstrated to be the main variable impacting bacterial communities in the Panlong Cave as indicated by redundancy analysis.Tax4fun2 analysis shows that microbes participated in carbon and nitrogen cycles also varied with niches.Microbes in dripping water,microbial biofilm on dry/wet surface of stalagmite were closely related to nitrogen cycle.On the contrast,those in weathered crust,sediments and cave wall were more involved in carbon fixation.Co-occurrence network analysis i

关 键 词：喀斯特洞穴 地下生物圈 碳固定 氮循环 共现网络 地质微生物 

分 类 号：X172[环境科学与工程—环境科学]