Multi-omics of Circular RNAs and Their Responses to Hormones in Moso Bamboo(Phyllostachys edulis)  

作　　者：Yongsheng Wang Huihui Wang Huiyuan Wang Ruifan Zhou Ji Wu Zekun Zhang Yandong Jin Tao Li Markus V.Kohnen Xuqing Liu Wentao Wei Kai Chen Yubang Gao Jiazhi Ding Hangxiao Zhang Bo Liu Chentao Lin Lianfeng Gu 

机构地区：[1]Basic Forestry and Proteomics Research Center,College of Forestry,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Fujian Agriculture and Forestry University,Fuzhou 350002,China [2]College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]Department of Molecular,Cell and Developmental Biology,University of California at Los Angeles,Los Angeles,CA 90095,USA

出　　处：《Genomics, Proteomics & Bioinformatics》2023年第4期866-885,共20页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31971734 and 31800566);the National Key R&D Program of China(Grant No.2021YFD2200505);the Distinguished Young Scholar Program of Fujian Agriculture and Forestry University(Grant No.xjq202017);the Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University(Grant No.324-1122yb061);the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University(Grant No.72202200205),China。

摘　　要：Circular RNAs(circRNAs)are endogenous non-coding RNAs with covalently closed structures,which have important functions in plants.However,their biogenesis,degradation,and function upon treatment with gibberellins(GAs)and auxins(1-naphthaleneacetic acid,NAA)remain unknown.Here,we systematically identified and characterized the expression patterns,evolutionary conservation,genomic features,and internal structures of circRNAs using RNase R-treated libraries from moso bamboo(Phyllostachys edulis)seedlings.Moreover,we investigated the biogenesis of circRNAs dependent on both cis-and trans-regulation.We explored the function of circRNAs,including their roles in regulating microRNA(miRNA)-related genes and modulating the alternative splicing of their linear counterparts.Importantly,we developed a customized degradome sequencing approach to detect miRNA-mediated cleavage of circRNAs.Finally,we presented a comprehensive view of the participation of circRNAs in the regulation of hormone metabolism upon treatment of bamboo seedlings with GA and NAA.Collectively,our study provides insights into the biogenesis,function,and miRNA-mediated degradation of circRNAs in moso bamboo.

关 键 词：Alternative splicing Circular RNA DEGRADOME Phyllostachys edulis PHYTOHORMONE 

分 类 号：S795.7[农业科学—林木遗传育种]