短期抗生素联用对氧化偶氮甲烷诱导小鼠肠道菌群的影响  

Effects of short-term antibiotics cocktail exposure on intestinal microbiota induced by azomethane in mice

作　　者：何夏梦 商正云 胡雯[1] 蒲芳芳 HE Xia-meng;SHANG Zheng-yun;HU Wen;PU Fang-fang(Department of Clinical Nutrition,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西医院临床营养科,四川成都610041 [2]重庆市江津区卫生健康委员会疾病预防控制科

出　　处：《现代预防医学》2024年第1期138-142,共5页Modern Preventive Medicine

基　　金：四川省科技厅重点研发项目(2019YFS0260)。

摘　　要：目的研究短期抗生素联用对氧化偶氮甲烷(azoxymethane,AOM)诱导小鼠肠道菌群的影响。方法40只3~4周龄ICR雄性小鼠随机等分成对照组(Control组)、AOM组(AOM组)、抗生素组(Antibiotics,Abx组)及抗生素+AOM组(AbxAOM组)。灌胃期:AbxAOM组和Abx组灌胃抗生素溶液,Control组和AOM组灌胃等体积纯水,每天2次,连续2周。腹腔注射期:AOM组和AbxAOM组腹腔注射AOM溶液,Control组和Abx组腹腔注射无菌0.9%NaCl溶液,每周1次,连续4周。腹腔注射结束5周后采集小鼠粪便,采用16S rRNA测序技术对粪便肠道菌群进行分析。结果AbxAOM组Chao1指数、ACE指数高于AOM组(P<0.05)。门水平下,AbxAOM组厚壁菌门相对丰度显著高于AOM组(P<0.05),拟杆菌门相对丰度显著低于AOM组(P<0.05)。属水平下,AbxAOM组拟杆菌属、拟普雷沃菌相对丰度较AOM组显著下降(P<0.05),另枝菌属、梭状杆菌XIVa、布劳特菌相对丰度显著增高(P<0.05)。结论抗生素处理后的肠道菌群改变提示可能由特定微生物组而非单种菌株对AOM诱导小鼠CRC的发生发展具有重要作用。Objective To study the effect of short-term antibiotics cocktail exposure on the intestinal flora induced by azomethane(AOM)in mice.Methods Forty male ICR mice aged 3 to 4 weeks were randomly divided into control group(Control group),AOM group(AOM group),antibiotic group(Abx group),and antibiotic+AOM group(AbxAOM group).During the gavage period,AbxAOM group and Abx group were administered with antibiotic solution,meanwile Control group and AOM group were administrated with corresponding volume of pure water twice a day for 2 weeks.During the period of intraperitoneal injection,AOM group and AbxAOM group were intraperitoneally injected with AOM solution,the Abx group and Control group were intraperitoneally injected with sterile 0.9%NaCl solution once a week for 4 weeks.The feces of mice were collected 5 weeks after intraperitoneal injection,and the intestinal flora of feces was analyzed by 16SrRNA sequencing.Results The Chao1 index and ACE index in AbxAOM group were higher than those in AOM group.Compared with AOM group,the relative abundance of Firmicutes in AbxAOM group was significantly higher(P<0.05),while that of Bacteroidetes was significantly lower at the phylum level(P<0.05).Compared with AOM group,the relative abundance of Bacteroides and Prevotella in AbxAOM group decreased significantly(P<0.05),and the relative abundance of Alistipes,Clostridium XIVa and Blautia increased significantly at the genus level(P<0.05).Conclusion The changes of intestinal flora after antibiotic treatment suggest that specific microbial groups rather than single strains may play an important role in the occurrence and development of CRC induced by AOM in mice.

关 键 词：抗生素 氧化偶氮甲烷 结直肠癌 肠道菌群 

分 类 号：R735.3[医药卫生—肿瘤] R37[医药卫生—临床医学]