‘金湘玉’黄桃果实糖与类黄酮代谢及转录组测序分析  

Transcriptome Sequencing for Sugar and Flavonoid Metabolism in Prunus persica'Jinxiangyu'

作　　者：陈为峰 王春发 黄佳[1] 李杜 张良波 CHEN Wei-feng;WANG Chun-fa;HUANG Jia;LI Du;ZHANG Liang-bo(Hunan Horticultural Research Institute,Changsha 410125,PRC;Hengyang Academy of Agricultural Sciences,Hengyang 421100,PRC;Hengnan Hongxiang Breeding Professional Cooperative,Hengyang 421125,PRC)

机构地区：[1]湖南省园艺研究所,湖南长沙410125 [2]衡阳市农业科学院,湖南衡阳421100 [3]衡南鸿祥种养专业合作社,湖南衡阳421125

出　　处：《湖南农业科学》2023年第12期7-12,共6页Hunan Agricultural Sciences

基　　金：湖南省农业科技创新资金项目(2022CX113,2022CX84-09);湖南创新型省份建设专项(2022NK4226)。

摘　　要：采用高效液相色谱法、RNA-seq转录组测序等方法研究了‘金湘玉’黄桃(Prunus persica'Jinxiangyu')果实不同发育时期(花后20~90 d)可溶性糖组分及类黄酮物质的含量变化,筛选出调控果实可溶性糖与类黄酮化合物形成的关键基因。结果表明:花后60~85 d为‘金湘玉’果实品质形成的关键阶段,果实中可溶性糖、可溶性固形物、果糖、蔗糖含量在此期间明显上升;成熟果实糖含量以果糖和蔗糖为主;山奈素苷在果实中的含量较低,槲皮素苷含量在幼果期含量较高,随果实成熟含量降低;在果实中不含有花色苷化合物;参与类黄酮代谢的相关基因(ANS、DFR、F3H、FLS、4CL1等)在果肉中表达量较低;在果实发育过程中共鉴定到181个差异表达基因参与5条糖代谢途径,其中SDH基因表达水平较高且随着果实成熟而持续上调,主要促进果实发育后期果糖含量的积累;SPS1、SS与SS1基因表达水平随着果实成熟而持续上调,对蔗糖调控起着关键作用;SUS3、INVA基因在果实发育前期表达水平较高,主要促进蔗糖的降解。High performance liquid chromatography(HPLC)and RNA-seq were employed to study the changes of soluble sugars and flavonoids in the fruits of Prunus persica'Jinxiangyu'at different developmental stages(20–90 d post flowering)and screen out the key genes regulating the formation of soluble sugars and flavonoids in fruits.The results showed that 60–85 d after flowering was the key stage for the fruit quality formation,and the content of soluble sugars,soluble solids,fructose,and sucrose in the fruits increased significantly during this stage.The sugars in mature fruits were mainly fructose and sucrose.The content of kaempferol glycosides was low in fruits.The content of quercetin glycosides was higher in young fruits and decreased as fruits became mature.There was no anthocyanins in the fruits.The genes(e.g.,ANS,DFR,F3H,FLS,and 4CL1)involved in flavonoid metabolism showed low expression levels in fruit flesh.A total of 181 differentially expressed genes during fruit development were identified,and these genes participated in 5 glucose metabolic pathways.Among them,SDH showed a high expression level and up-regulated expression as the fruits became mature,which promoted the accumulation of fructose in late fruit development.In addition,the fruit maturity was accompanied by the up-regulated expression of SPS1,SS,and SS1,which played a key role in sucrose regulation.The high expression levels of SUS3 and INVA in the early stage of fruit development promoted the degradation of sucrose.

关 键 词：黄桃 糖代谢 类黄酮化合物 差异基因表达 

分 类 号：S662.1[农业科学—果树学] Q811.4[农业科学—园艺学]