地生弯颈霉C41-3产低温脂肪酶发酵条件优化  

Optimization of Fermentation Condition of Cold⁃activer Lipase Producing by Tolypocladium geodes C41⁃3

作　　者：孙蕊 苏丹 张文浩 吕国忠 SUN Rui;SU Dan;ZHANG Wen-hao(College of Life Science and Engineering,Shenyang University,Shenyang,Liaoning 110044)

机构地区：[1]沈阳大学生命科学与工程学院,辽宁沈阳110044 [2]大连民族大学环境与资源学院,辽宁大连116600

出　　处：《安徽农业科学》2024年第2期1-4,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金面上项目(31770025);辽宁省教育厅青年育苗项目(2020JYT02);辽宁省自然科学基金指导计划项目(2019-ZD-0549);大连民族大学生物技术与资源利用教育部重点实验室开放课题(KF2021002)。

摘　　要：[目的]优化地生弯颈霉C41-3产低温脂肪酶发酵条件,为扩大低温脂肪酶的应用提供理论依据。[方法]使用棕榈酸对硝基苯酯(p-NPP)比色法测定脂肪酶的活性。[结果]最佳发酵培养基为胰蛋白胨40 g/L、α-乳糖20 g/L、MnSO_(4)·H_(2)O 1 g/L、NH_(4)NO_(3)1 g/L、乳化芥花油20 mL;最佳发酵条件为pH 5、发酵温度15℃、培养时间4 d。优化后,地生弯颈霉C41-3的脂肪酶活性从199.40 U/mL增加至5700.72 U/mL,脂肪酶活性增加了约27.6倍。[结论]该新型脂肪酶具有潜在的工业应用价值。[Objective]To optimize the fermentation conditions for low⁃activer lipase production by Tolypocladium geodes C41⁃3 and provide theoretical basis for expanding the application of low⁃activer lipase.[Method]The lipase activity was measured by the p⁃nitrophenyl palmitate(p⁃NPP)colorimetric method.[Result]Best fermentation medium was tryptone 40 g/L,α⁃lactose 20 g/L,MnSO_(4)·H_(2)O 1 g/L,NH_(4)NO_(3)1 g/L,emulsion canola oil 20 mL.Best fermentation condition was pH 5,fermentation temperature of 15℃,culture time of 4 days.After optimi⁃zation,the lipase activity of T.geodes C41⁃3 could be increased from 199.40 U/mL to 5700.72 U/mL,and the lipase activity increased by about 28.5 times,[Conclusion]The novel lipase has potential industrial applications value.

关 键 词：低温脂肪酶 地生弯颈霉C41-3 发酵条件优化 

分 类 号：Q949.32[生物学—植物学]