牦牛源短小芽胞杆菌TS1的分离鉴定及其生物学特性研究  

Isolation,identification and biological characterization of a strain of Bacillus pumilus TS1 from yak cattle

作　　者：刘银坤 李昊 李子昕 李知新 张玉彦 李文财 任建军 唐姝[1] LIU Yinkun;LI Hao;LI Zixin;LI Zhixin;ZHANG Yuyan;LI Wencai;REN Jianjun;TANG Shu(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Ningxia Animal Disease Prevention and Control Center,Yinchuan 750199,China;Ningxia Anlisen Biotechnology Co.Ltd.,Yinchuan 750001,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]宁夏动物疾病预防控制中心,宁夏银川750199 [3]宁夏安利森生物科技有限公司,宁夏银川750001

出　　处：《南京农业大学学报》2024年第1期69-77,共9页Journal of Nanjing Agricultural University

基　　金：宁夏自然科学基金项目(2022AAC02072);宁夏重点研发项目黄河生态流域生态环境保护与高质量发展科技支撑专项(21BEF02019)。

摘　　要：[目的]本试验旨在研究一株牦牛源短小芽孢杆菌(命名:TS1)的分离鉴定、抗逆性、对常用抗菌药物的耐药性以及对常见致病菌的抑菌活性,初步探索其作为益生菌添加剂在畜牧养殖中应用的可行性。[方法]对健康牦牛新鲜粪便进行100℃水浴处理10 min以筛选具有抗逆性的芽胞杆菌,进行形态学、生理生化特征及16S rRNA序列分析鉴定,通过测量生长曲线、耐酸、耐胆盐试验、药敏试验及抑菌试验等方法对其生物学特性进行研究,通过全基因测序分析其可能产生抗菌物质的类型。[结果]TS1为短小芽胞杆菌且具有耐酸、耐胆盐生长特性;TS1对于12种常见的抗菌药物敏感,且对大肠杆菌、沙门氏菌、金黄色葡萄球菌、猪链球菌具有抑菌作用。测序分析显示该菌株可编码与Amylocyclicin同源性达48.90%的一种新型抗菌肽,进而发挥其抗菌活性。[结论]短小芽胞杆菌TS1有一定的抗逆性和潜在的益生能力及广谱抗菌效果,并且在畜禽肠道微生物系统中对抗生素耐药性传递的风险较低,适合作为益生菌添加剂类抗生素替代产品。[Objectives]The aim of this experiment was to study the isolation and identification,resistance to common antimicrobial drugs and antibacterial activity of a strain of Bacillus subtilis of yak origin(named:TS1),and to explore the feasibility of its application as a probiotic additive in animal husbandry.[Methods]The fresh manure of healthy cattle was treated with water bath at 100 ℃ for 10 min to screen the resistant Bacillus,and then the morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis were integrated to identify them,and their biological characteristics were studied by measuring growth curves,acid and bile salt resistance tests,drug sensitivity tests and inhibition tests,and their possible production of the types of antibacterial substances they may produce.[Results]TS1 was Bacillus pumilus with acid-resistant and bile salt-resistant growth characteristics;TS1 was sensitive to 12 common antibacterial drugs and had inhibitory effects on Escherichia coli,Salmonella,Staphylococcus aureus and Streptococcus suis.Sequencing analysis showed that this strain could encode a novel antimicrobial peptide with 48.90% homology to Amylocyclicin,and thus exerted its antimicrobial activity.[Conclusions]This Bacillus pumilus TS1 has certain resistance and potential probiotic ability and broad-spectrum antibacterial effect,and has low risk of antibiotic resistance transmission in the livestock and poultry intestinal microbial system,and is suitable as a probiotic additive class antibiotic replacement product.

关 键 词：短小芽胞杆菌 分离鉴定 抑菌 抗生素替代品 益生菌添加剂 

分 类 号：S852.6[农业科学—基础兽医学]