The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa  

作　　者：Mercedes González-Martínez Pascual Sánchez-Martín Carmen López-Fernández Stephen D.Johnston Jaime Gosálvez 

机构地区：[1]Clínica Ginemed,Unidad de Reproducción,Sevilla,Spain [2]Department of Biology,Universidad Autónoma de Madrid,Cantoblanco,Madrid,Spain [3]School of Environment,The University of Queensland,Gatton,Australia [4]School of Veterinary Science,The University of Queensland,Gatton,Australia

出　　处：《Asian pacific Journal of Reproduction》2024年第1期22-27,共6页亚太生殖杂志（英文版）

摘　　要：Objective:To determine the relationship between teratozoospermia and sperm DNA fragmentation(SDF)in the human ejaculate.Methods:This retrospective study included 100 normozoospermic men as a control cohort(abnormal forms>14%),210 patients with a high level of abnormal forms(≤4%)and 65 patients presenting with a moderate level of abnormal forms(>4%to≤14%)based on the World Health Organization definitions.Sperm morphology was assessed using bright field microscopy.Sperm DNA fragmentation was assessed using the sperm chromatin dispersion assay.Non-parametric analyses were conducted to examine the relationship between abnormal sperm morphology and sperm DNA fragmentation;receiver operating characteristic(ROC)analyses were conducted to assess sensitivity and specificity of this relationship.Results:A correlation analysis revealed that the higher the proportion of abnormal spermatozoa in the ejaculate,the higher the level of SDF(Spearman's Rho=-0.230;P<0.001).Significant differences in the proportion of SDF were found when all cohorts were compared(P<0.001);these significant differences were also retained when the different cohorts were compared pairwise.ROC analysis showed a moderate but significant predictive value for SDF to differentiate patients with different levels of teratozoospemia.Conclusions:Although analysis of a more continuous range of values for teratozoospermia would help further clarify any causal relationship with SDF,there is clearly a synergistic or coincident affiliation between these variables that needs to be acknowledged by the clinician when interpreting the spermiogram.

关 键 词：TERATOZOOSPERMIA Sperm morphology Sperm DNA fragmentation Male factor Human reproduction Human fertility 

分 类 号：R698.2[医药卫生—泌尿科学]