Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrumβ-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia  

作　　者：Nurul Syazrah Anuar Hazmin Hazman Sharven Raj Jeyakumar Mohd Nasir Mohd Desa Hasni Idayu Saidi Siti Norbaya Masri Nur Afiza Aziz Nurshahira Sulaiman 

机构地区：[1]Department of Biomedical Science,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400,UPM Serdang,Selangor,Malaysia [2]Department of Medical Microbiology,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400,UPM Serdang,Selangor,Malaysia [3]Microbiology Unit,Department of Pathology,Hospital Sultanah Aminah,Jalan Persiaran Sultan Abu Bakar,80100 Johor Bahru,Johor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2024年第1期30-38,共9页亚太热带医药杂志（英文版）

基　　金：supported by the Ministry of Higher Education under the Fundamental Research Grant Scheme(FRGS/1/2021/SKK0/UPM/02/8);the Universiti Putra Malaysia Research University Grant Scheme(GP/IPS/2021/9702000).

摘　　要：Objective:To determine the distribution,phenotypic and genetic background of extended spectrumβ-lactamases(ESBL)-producing Klebsiella(K.)pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia.Methods:A total of 192 K.pneumoniae isolates were collected and subjected to antibiotic susceptibility,hypermucoviscosity test and multiplex PCR to detect the presence of K1-and K2-serotype associated genes.Multilocus sequence typing(MLST)was performed on ESBL-producing K.pneumoniae isolates presented with K1 and K2 serotypes,followed by phylogenetic analysis.Results:A total of 87 out of 192(45.3%)of the K.pneumoniae isolates collected were ESBL producers.However,only 8.3%(16/192)and 10.9%(21/192)of the total isolates were detected to carry K1-and K2-serotype associated genes,respectively.Statistical analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype(P=0.196).However,they were significantly associated with hypervirulent,as demonstrated by the positive string test(P<0.001).MLST analysis revealed that ST23 as the predominant sequence type(ST)in the K1 serotype,while the ST in the K2 serotype is more diverse.Conclusions:Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low,their coexistence warrants the need for continuous surveillance.MLST showed that these isolates were genetically heterogeneous.

关 键 词：Extended spectrumβ-lactamases Klebsiella pneumoniae Capsular serotypes GENOTYPIC 

分 类 号：R742.9[医药卫生—神经病学与精神病学]