LPS致家兔发热模型的建立及用于壮瑶药解热活性成分筛选  

Establishment of LPS-induced rabbit fever model and evaluation of antipyretic effects of Zhuang and Yao medicine ingredients

作　　者：邓玲玉 朱华[1] 许立拔 谢凤凤 罗静 平静云 农丽珍 李茵兰 曾艳芳 陈雅玲 岑思 DENG Ling-yu;ZHU Hua;XU Li-ba;XIE Feng-feng;LUO Jing;PING Jing-yun;NONG Li-zhen;LI Yin-lan;ZENG Yan-fang;CHEN Ya-ling;CEN Si(Guangxi Scientific Research Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学,广西医药科学实验中心,南宁530200

出　　处：《湖北农业科学》2023年第12期120-129,共10页Hubei Agricultural Sciences

基　　金：广西重点研发计划项目(桂科AB21196016);广西科技基地和人才专项(桂科AD21238031);广西壮瑶药重点实验室(桂科基字[2014]32号);壮瑶药协同创新中心(桂教科研[2013]20号);广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号);广西中医药重点学科壮药学(GZXK-Z-20-64);国家自然科学基金项目(82060695);广西一流学科中药学(民族药学)(桂教科研[2018]12号)。

摘　　要：通过静脉注射不同剂量内毒素(LPS),评价LPS所致家兔发热模型的最佳剂量。造模后1 h,所有提取物、壮瑶药活性单体进行给药。使用智能热原仪测量LPS家兔的肛温变化,并计算肛温增长值、变化率和每个时间点解热积分及各供试品的解热总积分。结果表明,注射LPS 1 h后,所有家兔肛温均比基础肛温升高0.8~3.0℃,模型组家兔在造模后肛温维持高温状态,未见明显改变。与模型组比较,7种壮瑶药的提取物均有1个或以上时间点肛温变化率显著降低,按解热总积分大小排序表现为蝙蝠草醇提物>旱田草水部位提取物>白子菜水提物>火炭母醇提物>旱田草石油醚部位提取物>拳卷地钱醇提物>兰香草水提物>旱田草正丁醇部位提取物>铁皮石斛榨汁。13种壮瑶药的活性单体均有1个或以上时间点肛温变化率显著降低,按解热总积分大小排序表现为山奈酚>川陈皮素>橙皮苷>异槲皮素>芹菜素>槲皮素>紫云英苷>没食子酸>醋酸洗必泰>鞣花酸>绿原酸>木犀草素>金合欢素。在本试验条件下,确定LPS复制家兔发热模型的最佳剂量为4.0μg/kg;7种壮瑶药提取物和13种壮瑶药活性单体有良好的解热作用。The optimal dose of replicating the fever model of endotoxin(LPS)in rabbits was evaluated using different doses of LPS in⁃travenously.1 h after modeling,all extracts and active monomers of Zhuang and Yao ethnic medicine were administered.The rectal temperature changes of LPS rabbits were measured by the intelligent pyrogen instrument,and the anal temperature growth value,change rate,antipyretic score at each time point and the total antipyretic score of each test sample were calculated.The results showed that after LPS injection for 1 h,the temperature of all rabbits increased by 0.8~3.0℃compared with the basal rectal temperature,and the temperature of the model rabbits remained high after molding,and there was no significant change.Compared with the model group,the rectal temperature change rate of rabbits treated with 7 kinds of extracts from Zhuang and Yao ethnic medicine was signifi⁃cantly reduced in 1 or more time points,and the order of the total heat antipyretic score was as follows:Ethanolic extract of Christia vespertilionis(L.f.)Bahn.f.>water extract of Lindernia ruellioides(Colsm.)Pennell.>water extract of Gynura divaricata(Linn.)DC.>ethanolic extract of Polygonum chinense L.>petroleum ether extract of Lindernia ruellioides(Colsm.)Pennell.>ethanolic extract of Marchantia convluta Gao et Chang>water extract of Caryopteris incana(Thunb.)Miq.>n-butanol extract of Lindernia ruellioides(Col⁃sm.)Pennell.>water extract of Dendrobium officinale Kimura et Migo.The change rate of rectal temperature of rabbits treated with 13 active monomers of Zhuang and Yao ethnic medicine decreased significantly at one or more time points.According to the total score of antipyretic effects,the order was as follows:Kaempferol>nobiletin>hesperidin>isoquercetin>apigenin>quercetin>astragalin>gallic acid>chlorhexidine acetate>ellagic acid>chlorogenic acid>luteolin>acacetin.Under the conditions of this experiment,the optimal dose of LPS replicating the fever model of rabbits was 4.0μg/kg;the extracts obtained from 7 kinds o

关 键 词：发热 内毒素(LPS) 壮瑶药 活性单体 解热作用 

分 类 号：R363[医药卫生—病理学] R285.6[医药卫生—基础医学]