HPLC-ELSD法测定梅子提取物中糖和糖醇的含量  

Determination of sugar and sugar alcohol content in plum extract by HPLC-ELSD

作　　者：马舒翼 梁佳欣 许春平 张婷 李冉[2] 董爱君[2] 肜霖[2] MA Shu-yi;LIANG Jia-xin;XU Chun-ping;ZHANG Ting;LI Ran;DONG Ai-jun;RONG Lin(College of Tobacco Science and Engineering,Zhengzhou University of Light Industry,Zhengzhou 450000,China;China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430040,China;School of Pharmacentical Sciences,South-Central University Minzu,Wuhan 430074,China)

机构地区：[1]郑州轻工业大学烟草科学与工程学院,郑州450000 [2]湖北中烟工业有限责任公司,武汉430040 [3]中南民族大学药学院,武汉430074

出　　处：《湖北农业科学》2023年第12期158-162,共5页Hubei Agricultural Sciences

基　　金：河南省科技创新团队计划项目(20IRTSTHN022);湖北中烟工业有限责任公司科技项目(2016JCYL3GC2B005)。

摘　　要：建立了一种高效液相色谱-蒸发光散射法(HPLC-ELSD)同时测定梅子提取物中果糖、葡萄糖、山梨醇、蔗糖、麦芽糖水合物、麦芽三糖、麦芽四糖的方法。分离采用Grace Prevail Carbohydrate ES色谱柱,柱温为30℃;流动相为乙腈-水,流速为1.00 mL/min;检测使用蒸发光散射检测器,漂移管温度为60℃,氮气流速为2.50 L/min。结果表明,果糖、山梨醇、葡萄糖、蔗糖、麦芽糖水合物、麦芽三糖、麦芽四糖分别在34.69~2 220.00、16.22~1 038.00、38.66~2 474.00、24.53~1 570.00、22.91~1 466.00、6.09~390.00、14.69~940.00μg/mL范围内线性关系良好,精密度良好,重复性的相对标准偏差(RSD)为1.00%~4.09%,各目标物的回收率为85.5%~110.1%,精密度为0.3%~4.6%(n=6)。本方法在30 min内实现了糖和糖醇的分离,灵敏度和重复性检测较好,有效避免了溶剂及温度的影响,测量结果较准确且操作方便,可用于批量检测梅子提取物中糖和糖醇的含量。A high performance liquid chromatography and evaporative light scattering detector(HPLC-ELSD)method was estab⁃lished for the simultaneous determination of fructose,glucose,sorbitol,sucrose,maltose hydrate,maltotriose,and maltotriose in plum extract.The separation was carried out using a Grace Prevail Carbohydrate ES column at a column temperature of 30℃;the mo⁃bile phase was acetonitrile water,with a flow rate of 1.00 mL/min;the detection used an evaporative light scattering detector,with a drift tube temperature of 60℃and a nitrogen flow rate of 2.50 L/min.The results showed that fructose,sorbitol,glucose,sucrose,maltose hydrate,maltotriose,and maltotetraose had a good linear relationship,ranging from 34.69~2220.00,16.22~1038.00,38.66~2474.00,24.53~1570.00,22.91~1466.00,6.09~390.00 and 14.69~940.00μg/mL,respectively,the precision was good,and the relative standard deviation(RSD)of repeatability was 1.00%~4.09%,the recovery rates of each target substance were 85.5%~110.1%,and the precision was 0.3%~4.6%(n=6).This method achieved the separation of sugar and sugar alcohols within 30 min⁃utes,with good sensitivity and repeatability detection,effectively avoiding the influence of solvents and temperature.The measure⁃ment results were accurate and the operation was easy,which could be used for batch detection of sugar and sugar alcohols in plum extract.

关 键 词：高效液相色谱(HPLC) 蒸发光散射检测器(ELSD) 梅子提取物 糖 糖醇 

分 类 号：TS411[农业科学—烟草工业]