褪黑素对创伤后应激障碍大鼠下丘脑-垂体-肾上腺轴的影响  

The effects of melatonin therapy on hypothalamic-pituitary-adrenalaxis in rats with posttraumatic stress disorder

作　　者：孙霄 李志达[2] 郝广志 董玉书 SUN Xiao;LI Zhida;HAO Guangzhi;DONG Yushu(Department of Neurosurgery,General Hospital of Northern Theater Command of Chinese People’s Liberation Army,Shenyang 110016;Department of Medical Imaging Technology,School of Medcine,Hebi Polytechnic,Hebi 458030,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016 [2]鹤壁职业技术学院医学院医学影像技术教研室,鹤壁458030

出　　处：《神经解剖学杂志》2023年第6期677-681,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071481)。

摘　　要：目的:探讨褪黑素(MLT)对足部电击所致创伤后应激障碍(PTSD)大鼠下丘脑-垂体-肾上腺(HPA)轴的影响。方法:利用足底电击法制备大鼠PTSD模型,通过腹腔注射方法给予治疗组大鼠MLT。通过拒俘反应测试检测大鼠的行为学变化,利用real time RT-PCR方法检测下丘脑中促肾上腺皮质激素释放激素(CRH)mRNA的表达,利用酶联免疫吸附试验(ELISA)检测血清中促肾上腺皮质激素(ACTH)、肾上腺素(EPI)和糖皮质激素(GC)的含量。结果:PTSD组大鼠拒俘反应明显(P<0.05),下丘脑中CRH mRNA表达升高(P<0.05),血清中ACTH和EPI明显升高(P<0.05),但是GC水平下降(P<0.05)。MLT治疗后可以明显缓解PTSD大鼠拒俘反应(P<0.05),同时降低下丘脑中CRH mRNA表达(P<0.05),降低血清中ACTH和EPI水平并升高GC的水平(P<0.05)。结论:MLT治疗可缓解PTSD大鼠的症状,并恢复HPA轴的神经内分泌平衡。Objective:To investigate the effects of melatonin(MLT)on hypothalamic-pituitary-adrenal(HPA)axis in posttraumatic stress disorder(PTSD)rats induced by foot shocks.Methods:The rat model of PTSD was prepared by plantar electroshock,and MLT was given by intraperitoneal injection.The behavioral changes of rats were detected by rejection reaction test,the mRNA expression of corticotropin-releasing hormone(CRH)in hypothalamus was detected by real time RT-PCR,and the contents of adrenocorticotropin hormone(ACTH),epinephrine(EPI)and glucocorticoid(GC)in serum were detected by enzyme-linked immunosorbent assay(ELISA).Results:In the PTSD group,the rejection reaction was obvious,the expression of CRH mRNA in hypothalamus was increased(P<0.05),the serum ACTH and EPI were increased(P<0.05),but the GC level was decreased(P<0.05).After MLT treatment,the rejection reaction of PTSD rats was significantly alleviated,CRH mRNA expression in hypothalamus was decreased(P<0.05),serum ACTH and EPI levels were decreased(P<0.05),and GC levels were increased(P<0.05).Conclusion:MLT treatment can relieve the symptoms of PTSD and restore the neuroendocrine balance of HPA axis in rats.

关 键 词：创伤后应激障碍 褪黑素 下丘脑-垂体-肾上腺轴 促肾上腺皮质激素释放激素 促肾上腺皮质激素 肾上腺素 糖皮质激素 

分 类 号：R749.5[医药卫生—神经病学与精神病学]