可控性运动皮质撞击损伤大鼠模型的制备与运动功能障碍的检测  

Establishment controlled motor cortex impact injury model ofrats and detection of motor dysfunction

作　　者：宋伟龙 郝广志 王振 金海 SONG Weilong;HAO Guangzhi;WANG Zhen;JIN Hai(Department of Neurosurgery,General Hospital of Northern Theater Command of Chinese People’s Liberation Army,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2023年第6期705-708,共4页Chinese Journal of Neuroanatomy

基　　金：辽宁省应用基础研究计划(2022JH2/101500037)。

摘　　要：目的:本研究旨在制备可控性运动皮质撞击损伤(CCI)大鼠模型。方法:利用电磁冲击法制备SD大鼠CCI模型,利用ELISA法检测血清中神经元特异性烯醇化酶(NSE)的变化。利用神经功能缺损评分(NSS)评估大鼠神经功能障碍,利用贴纸去除试验检测大鼠感觉运动功能改变,利用食物抓取试验检测大鼠精细运动能力。结果:CCI损伤后1 h大鼠血清中NSE值升高,6 h后达到最高;CCI模型大鼠感觉运动功能和精细运动功能明显受损。结论:成功制备了大鼠运动皮质的CCI模型,大鼠显示出明显的运动功能障碍。Objective:This study was to establish a rat model of controlled motor cortex impact injury(CCI).Methods:SD rat CCI model was prepared by electromagnetic shock method.A neurologic severity score(NSS)was employed to evaluate the status of the rat after injury.The changes of neuron-specific enolase(NSE)in serum were detected by ELISA.The sensomotor function was detected by sticker removal test,and the fine motor function was detected by food pellets grasping test.Results:The serum NSE level of rats increased 1 h after CCI trauma,and reached the highest level 6 h after CCI trauma.The sensomotor function and fine motor function of CCI model rats were significantly impaired.Conclusion:The CCI model of rat motor cortex was successfully established,and the rats showed obvious motor dysfunction.

关 键 词：可控性运动皮质撞击损伤 神经元特异性烯醇化酶 神经功能评分 贴纸去除试验 食物抓取试验 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]