基于Nrf-2通路探讨蛭龙活血通瘀胶囊对脑出血大鼠氧化应激损伤的机制  

Mechanism investigation of Zhilong Huoxue Tongyu Capsule on oxidative stress injury in rats with cerebral hemorrhage based on Nrf-2 pathway

作　　者：梁俊杰 杨燕 刘天助 徐厚平 刘平 张蕾 刘孟楠 杨思进 LIANG Junjie;YANG Yan;LIU Tianzhu;XU Houping;LIU Ping;ZHANG Lei;LIU Mengnan;YANG Sijin(Department of Cardiovascular Medicine,The Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000,China;Department of Neurology,The Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000,China;National Clinical Research Base of Traditional Chinese Medicine,The Affiliated Traditional Chinese Medicine Hospi-tal,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医医院心血管内科,泸州646000 [2]西南医科大学附属中医医院神经内科,泸州646000 [3]西南医科大学附属中医医院,国家中医临床研究基地,泸州646000

出　　处：《西南医科大学学报》2024年第1期45-50,共6页Journal of Southwest Medical University

基　　金：四川省科技计划重点研发项目(2019YFS0543);四川省中医药管理局基金项目(2020ZD002)。

摘　　要：目的探究蛭龙活血通瘀胶囊治疗脑出血氧化应激损伤的机制。方法将45只清洁级SD大鼠随机均分为假手术组(Sham组),脑出血模型组(ICH组),蛭龙活血通瘀胶囊组(ZL组)。ICH组及ZL组以自体血注入法造模,造模成功后,ZL组以蛭龙活血通瘀胶囊(1g∙kg-1∙d-1)灌胃,ICH组及Sham组以等剂量生理盐水灌胃,喂养7 d后,观察各组大鼠神经功能缺损程度,检测脑组织及血清内氧化因子与抗氧化因子的表达,检测脑海马组织中抗氧化应激相关蛋白表达情况。结果与Sham组相比,ICH、ZL组有明显神经功能缺损,脑组织及血清中抗氧化因子含量降低,氧化因子含量增加,脑组织中核因子E2相关因子2(nuclear factor E2-related factor 2,Nrf-2)相关通路蛋白表达程度升高;与ICH组相比,ZL的神经功能缺损程度减轻,脑组织及血清中抗氧化因子含量增加,氧化因子含量减少,脑组织中Nrf-2相关通路蛋白表达程度更高。结论蛭龙活血通瘀胶囊能减轻脑出血大鼠的神经功能损伤,缓解脑组织病变,提高大鼠脑出血后Nrf-2相关蛋白表达及脑组织抗氧化能力,减轻脑出血后的氧化应激损伤。Objective To explore the mechanism of Zhilong Huoxue Tongyu capsules in treating oxidative stress injury of cere-bral hemorrhage.Methods The total of 45 SD rats were randomly divided into sham operation group(Sham group),intracerebral hem-orrhage model group(ICH group),and Zhilong Huoxue Tongyu capsule administration group(ZL group).ICH group and ZL group were modeld with autologous blood injection method.After successful establishments of the models,ZL group was given Zhilong Huoxue Tongyu capsule(1 g·kg-1·d-1)by gavage,ICH group and ZL group were given an equal dose of normal saline by gavage.After 7 days of feeding,the degrees of neurological impairment in rats in each group were observed,the expression of oxidation factors and antioxidant factors in brain tissue and serum were detected,and the expression of antioxidant stress related proteins in hippocampus tissue were de-tected.Results Compared with the sham group,the ICH and ZL groups showed more severe neurological deficits,lower levels of antioxi-dant factors in brain tissue and serum,higher levels of oxidative factors,and higher protein expression of nrf-2-related pathways in brain tissue;Compared with the ICH group,ZL group exhibited a reduced degree of neurological deficit,an increased content of antioxi-dant factors in brain tissue and serum,a reduced content of oxidative factors,and a higher degree of nrf-2-related pathway protein ex-pression in brain tissue.Conclusion Zhilong Huoxue Tongyu capsules could reduce nerve function injury and brain tissue lesion in rats with cerebral hemorrhage,improve the expression of Nrf-2 related protein,improve the antioxidant capacity of brain tissue,and reduce the oxidative stress injury after cerebral hemorrhage.

关 键 词：蛭龙活血通瘀胶囊 氧化应激 核因子E2相关因子2 脑出血 

分 类 号：R743.34[医药卫生—神经病学与精神病学]