龙芽草总黄酮的闪式提取工艺优化及提取物的抑菌和抗炎活性研究  

Optimization of flash extraction process of Agrimonia pilosa total flavonoids and antibacterial and anti-inflammatory activities of the extract

作　　者：杨佳 金哲勇 姜君[1] 金英花[1] YANG jia;JIN Yongzhe;JIANG Jun;JIN Yinghua(Agricultural College of Yanbian University,Yanji Jilin 133002,China;Experimental Animal Center of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边大学实验动物中心,吉林延吉133002

出　　处：《延边大学农学学报》2023年第4期16-25,共10页Agricultural Science Journal of Yanbian University

基　　金：吉林省科技发展计划项目(20210101231JC);吉林省教育厅“十三五”科学技术项目(JJKH20200523KJ)。

摘　　要：为优化龙芽草总黄酮闪式提取工艺,以龙芽草全株为试验材料,以总黄酮含量为指标,采用响应面法对提取时间、提取液料比、提取溶剂浓度进行了优化,结果表明:当提取时间为41.09 s,提取液料比为40.19∶1(mL∶g),提取溶剂浓度为41.37%时,总黄酮含量最高,可达到94.25 mg/g。为了将龙芽草提取物应用于产品生产中,对其提取物进行了抑菌及抗炎活性的研究。抑菌试验结果表明,龙芽草提取物对6种致病菌均表现出良好的抑菌活性,其中对蜡样芽孢杆菌的抑菌效果最佳,最低抑菌浓度为4 mg/mL。在抗炎试验中,建立了以脂多糖(LPS)诱导的小鼠单核巨噬细胞(Raw 264.7)炎症模型,调查了提取物对NO、iNOS和COX-2炎症指标的影响。结果表明:经过提取物处理后的LPS诱导的Raw 264.7细胞的炎症指标呈剂量依赖性地下降。因此,该研究旨在为龙芽草进一步的开发和利用提供一定理论基础。To optimize a flash extraction process of Agrimonia pilosa total flavonoids,the whole plants of A.pilosa were used as the experimental material to investigate the optimization conditions of flash extraction by controlling the extract time,liquid to material ratio,and solvent(methanol)concentration,using the total flavonoid content as the evaluation index.The results showed that the highest total flavonoid content reached 94.25 mg/g when the extraction time was 41.09 s,the extract liquid to material ratio was 40.19∶1(mL∶g),and the extraction solvent concentration was 41.37%.Furthermore,the antibacterial and anti-inflammatory activities were investigated to use the extract of A.pilosa for the application in the production of products.The results of the antibacterial experiment showed that the extract obviously inhibited the growth of six tested pathogenic bacteria,among which the highest antibacterial activity was observed against Bacillus cereus,with the minimum inhibitory concentration of 4 mg/mL.In the anti-inflammatory experiment,a lipopolysaccharide(LPS)-induced mouse monocyte-macrophage(Raw 264.7)inflammation model was established,and the effects of the extract on levels of inflammatory mediators,i.e.,NO,iNOS,and COX-2,were investigated.The results showed that after treatment with the extract,the LPS-induced inflammatory mediators in Raw 264.7 cells decreased in a dose-dependent manner.The findings of this study provided theoretical basis for the further development and use of A.Pilosa.

关 键 词：龙芽草总黄酮 闪式提取法 抑菌活性 炎症因子 

分 类 号：R284.1[医药卫生—中药学]