依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控  

Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway

作　　者：祁志 杨力 贾秋叶 陈浩伦 段兆达 吴春云 Qi Zhi;Yang Li;Jia Qiuye;Chen Haolun;Duan Zhaoda;Wu Chunyun(Department of Human Anatomy and Tissue Embryology,School of Basic Medical Sciences,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明650500

出　　处：《中国临床解剖学杂志》2024年第1期47-53,共7页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(81960223);云南省科技厅昆明医科大学联合专项(202301AY070001-163);云南省大学生创新性实验计划项目;昆明医科大学大学生创新性实验计划项目(2022JXD301)。

摘　　要：目的探究依达拉奉对LPS激活的小胶质细胞Sirt3和MAPKs通路相关蛋白的作用及其调控机制。方法采用Western blot和免疫荧光双标染色检测LPS激活的BV2小胶质细胞中Sirt3和MAPKs通路相关蛋白ERK1/2、P38、JNK及p-ERK1/2、p-P38和p-JNK的表达;检测ERK1/2通路抑制剂处理后p-ERK1/2和Sirt3的表达。结果LPS激活的BV细胞中Sirt3、p-ERK1/2、p-P38和p-JNK表达显著增高,依达拉奉能促进Sirt3和p-ERK1/2过表达,并降低p-P38和p-JNK表达;ERK1/2抑制后p-ERK1/2和Sirt3的蛋白表达降低。依达拉奉可促进激活的BV2细胞中Sirt3和p-ERK1/2过表达,并下调过表达的pP38和p-JNK。结论依达拉奉可调节BV2细胞中MAPKs信号通路并促进Sirt3生成,可能经ERK/Sirt3通路发挥抗炎作用。Objective To investigate the effect of edaravone on Sirt3 and MAPKs pathway-related proteins in LPS-activated microglia and its regulatory mechanism.Methods Western Blot and immunofluorescence were used to detect the effect of edaravone on the protein expression of Sirt3 and MAPKs pathway-related proteins ERK1/2,P38,JNK as well as their phosphorylated forms p-ERK1/2,p-P38 and p-JNK in the activated BV2 cells.Western Blot techniques were applied to detect the effects of ERK1/2 pathway inhibitor on the expressions of p-ERK1/2 and Sirt3.Results The expressions of Sirt3,p-ERK1/2,p-P38 and p-JNK in microglia were significantly increased after BV2 cells activation.Edaravone promoted the expression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38.The protein expression of Sirt3 and p-ERK1/2 decreased after co-treatment with edaravone and ERK1/2 inhibitor.Edaravone promoted the overexpression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38.Conclusions Edaravone can regulate MAPKs signaling pathway and promote Sirt3 production in BV2 cells,possibly regulates neuroinflammatory responses via ERK/Sirt3 signaling pathway.

关 键 词：依达拉奉 Sirt3 小胶质细胞 MAPKS信号通路 

分 类 号：R363.21[医药卫生—病理学]