经典型霍奇金淋巴瘤中CXCR5的表达及临床病理意义  

Expression of CXCR5 and its significance of clinical and pathological in classical Hodgkin lymphoma

作　　者：夏志雄 周小鸽[1] 郑媛媛[1] 张燕林[1] 谢建兰[1] XIA Zhixiong;ZHOU Xiaoge;ZHENG Yuanyuan;ZHANG Yanlin;XIE Jianlan(Department of Pathology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Pathology,Wuhan Central Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430010,China)

机构地区：[1]首都医科大学附属北京友谊医院病理科,北京100050 [2]华中科技大学同济医学院附属武汉中心医院病理科,武汉430010

出　　处：《临床与实验病理学杂志》2024年第1期35-39,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨经典型霍奇金淋巴瘤(classic Hodgkin lymphoma,CHL)中CXCR5的表达及意义。方法采用免疫组化EnVision两步法检测33例CHL中CXCR5的表达,分析CXCR5在CHL四种亚型中的表达情况及临床病理诊断意义;同时收集10例ALK阳性间变性大细胞淋巴瘤(anaplastic large cell lymphoma,ALCL)和10例ALK阴性ALCL作为对照组,对比分析CXCR5的表达情况。结果33例CHL中,31例CXCR5阳性(93.94%):其中结节硬化型15例(15/16,93.75%)、混合细胞型12例(12/13,92.31%)、淋巴细胞丰富型2例,淋巴细胞消减型2例。CHL中CXCR5的表达情况分别为:33例CD30阳性和PAX5弱阳性中31例阳性(93.94%);14例CD15阴性中12例阳性(85.71%);26例CD20阴性中24例阳性(92.31%);6例LMP1阴性中5例阳性;11例EBER阴性中10例阳性(90.91%)。对照组20例ALCL中,肿瘤细胞CXCR5均阴性。结论CHL中CXCR5阳性率较高,当肿瘤细胞中CD15、LMP1和CD20阴性,或EBER阴性时,CXCR5仍有较高的阳性率,有助于CHL的诊断。尤其是组织形态学和免疫表型不典型的CHL病例,可借助免疫组化标记CXCR5与ALCL鉴别。Purpose To investigate the expression of Chemokine(C-X-C Motif)receptor 5(CXCR5)and its clinicopathological significance in classic Hodgkin lymphoma(CHL).Methods The expression of CXCR5 was assessed in 33 patients by immunohistochemistry(IHC),and retrospectively analyzed the expression and clinical significance of CXCR5 in the four subtypes of CHL.Meanwhile,10 cases of ALK-positive anaplastic large cell lymphoma(ALCL)and 10 cases of ALK-negative ALCL were collected as the control group.Results There were 31 cases with CXCR5-positive in all 33 cases(93.94%),including 15/16(93.75%)in nodular sclerosis CHL,12/13(92.31%)in mixed cellularity CHL,2/2 in lymphocyte-rich CHL,and 2/2 in lymphocyte-depleted CHL.The positive expressions of CXCR5 in different immunophenotypes of CHL were as follow,31/33(93.94%)in CD30 positive and PAX5 weakly positive CHL.12/14(85.71%)in CD15 negative CHL,24/26(92.31%)in CD20 negative CHL,10/11(90.91%)in EBER-negative CHL and 5/6 in LMP1-negative CHL.CXCR5 were not expressed in all 20 cases of ALCL.Conclusion The positive expression rate of CXCR5 in CHL is high.When the tumor cells are negative for CD15,LMP1 and CD20 or EBER,CXCR5 also has a high positive expression rate,which is helpful for the diagnosis of CHL.CXCR5 can be used to differentiate CHL from ALCL,especially the cases lacking typical morphology and immunohistochemistry.

关 键 词：淋巴瘤 经典型霍奇金淋巴瘤 间变性大细胞淋巴瘤 CXCR5 免疫组织化学 

分 类 号：R733.4[医药卫生—肿瘤]