Rational Design and Cellular Synthesis of Proteins with Unconventional Chemical Topology  

作　　者：Tianzuo Li Fan Zhang Jing Fang Yajie Liu Wen-Bin Zhang 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Polymer Chemistry&Physics of Ministry of Education,Center for Soft Matter Science and Engineering,College of Chemistry and Molecular Engineering,Peking University,Beijing,100871 China [2]Beijing Academy of Artificial Intelligence,Beijing,100084 China

出　　处：《Chinese Journal of Chemistry》2023年第21期2873-2880,共8页中国化学（英文版）

基　　金：We are grateful for the financial support from the National Key R&D Program of China(No.2020YFA0908100);the National Natural Science Foundation of China(Nos.21991132,21925102,92056118,22101010,22201016,22201017);Beijing National Laboratory for Molecular Sciences(BNLMS-CXXM-202006)。

摘　　要：Chemical topology refers to the three-dimensional arrangement(i.e.,connectivity and spatial relationship)of a molecule's constituent atoms and bonds.The molecular mechanism for translation defines the linear configuration of all nascent proteins.Nontrivial protein topology arises only upon post-translational processing events and often imparts functional benefits such as enhanced stability,making topology a unique dimension for protein engineering.Utilizing the assembly-reaction synergy,our group has developed several methods for the effective and convenient cellular synthesis of a variety of topological proteins,such as lasso proteins,protein rotaxanes,and protein catenanes.The work opens the access to new protein classes and paves the road toward illustrating the topological effects on structure-function relationship of proteins,which lays solid foundation for exploring topological proteins’practical application.

关 键 词：Active template CATENANE Cellular synthesis Chemical topology Lasso protein Protein engineering 

分 类 号：O62[理学—有机化学]