SLC6A8通过BNIP3L调控线粒体自噬促进肝癌细胞增殖  

SLC6A8 promotes liver cancer cell proliferation by regulating mitochondrial autophagy through BNIP3L

作　　者：路远[1] 唐俊[1] 李文川[1] 许佐明[1] 罗宗将 马嘉盛 浦涧[1] LU Yuan;TANG Jun;LI Wen-chuan;XU Zuo-ming;LUO Zong-jiang;MA Jia-sheng;PU Jian(Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院附属医院,广西百色533000

出　　处：《海南医学院学报》2024年第2期88-93,99,共7页Journal of Hainan Medical University

基　　金：2021年度广西高校中青年教师科研基础能力提升项目(2021KY0539)。

摘　　要：目的:探讨SLC6A8通过BNIP3L调控线粒体自噬对肝癌细胞增殖的影响。方法:通过TCGA数据库分析SLC6A8基因在肝癌组织中的表达情况,并与BNIP3L表达进行相关性分析。使用RT-PCR和Western blot技术检测SLC6A8过表达人肝癌Huh-7及Hep3B细胞中SLC6A8和BNIP3L的表达。免疫荧光标记和CCK-8检测法用于观察SLC6A8过表达人肝癌Huh-7及Hep3B细胞线粒体自噬和细胞增殖率。使用CCK-8检测法评估在沉默BNIP3L后SLC6A8过表达人肝癌Huh-7及Hep3B细胞增殖率。结果:SLC6A8在肝癌组织中显著高表达,并与BNIP3L表达呈正相关。SLC6A8过表达可显著促进线粒体自噬和人肝癌Huh-7及Hep3B细胞的增殖。此外,SLC6A8过表达显著促进BNIP3L mRNA和蛋白的表达。沉默BNIP3L后,SLC6A8过表达对肝癌细胞增殖的促进作用被逆转。结论:SLC6A8在肝癌组织中的高表达与BNIP3L有正相关性,且SLC6A8的过表达能促进线粒体自噬和肝癌细胞的增殖,沉默BNIP3L可逆转SLC6A8过表达对肝癌细胞增殖促进作用。这为肝癌的治疗提供了新的靶点和策略。Objective:To investigate the effect of SLC6A8 on the proliferation of liver cancer cells by regulating mitophagy through BNIP3L.Methods:The expression of the SLC6A8 gene in liver cancer tissues was analyzed using the TCGA database,and its correlation with BNIP3L expression was assessed.RT-PCR and Western blot techniques were employed to detect the expression of SLC6A8 and BNIP3L in human liver cancer Huh-7 and Hep3B cells.Immunofluorescence labeling and CCK-8 assay were used to observe mitochondrial autophagy and cell proliferation rate in SLC6A8-overexpressing Huh-7 and Hep3B cells.CCK-8 detection method was used to evaluate the proliferation rate of SLC6A8-overexpressing Huh-7 and Hep3B cells after silencing BNIP3L.Results:SLC6A8 was significantly overexpressed in liver cancer tissues and positively correlated with BNIP3L expression.Overexpression of SLC6A8 significantly promoted mitochondrial autophagy and proliferation of Huh-7 and Hep3B cells.Additionally,SLC6A8 overexpression significantly enhanced the expression of BNIP3L mRNA and protein.Upon BNIP3L silencing,the proliferative effect of SLC6A8 overexpression on liver cancer cells was reversed.Conclusion:High expression of SLC6A8 in liver cancer tissues is positively correlated with BNIP3L,and overexpression of SLC6A8 promotes mitochondrial autophagy and liver cancer cell proliferation.Silencing BNIP3L can revese the effect of overexpression of SLC6A8 on liver cancer cell proliferation.This provides new targets and strategies for the treatment of liver cancer.

关 键 词：线粒体自噬 SLC6A8 BNIP3L 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]