莲NnOMT和NnNMT基因家族的鉴定与分析  

Identification and Analysis of NnOMT and NnNMT Gene Families in Nelumbonucifera

作　　者：陈竹荫 俞越童 郝晨阳 刘鸣茹 陈莎 CHEN Zhuyin;YU Yuetong;HAO Chenyang;LIU Mingru;CHEN Sha(Beijing Key Laboratory of Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,中药鉴定与安全性评估北京市重点实验室,北京100700

出　　处：《中国实验方剂学杂志》2024年第3期152-160,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(3217020858);中国中医科学院科技创新工程项目(CI2021A04108)。

摘　　要：目的:莲苄基异喹啉生物碱(BIAs)的生物合成途径的解析具有重要的理论和经济价值,为了更好地促进莲BIAs生物合成途径的解析,本研究对莲氧甲基转移酶(NnOMT)、莲氮甲基转移酶(NnNMT)基因家族进行鉴定和生物信息学分析。方法:在莲全基因组的基础上,利用UniPort、美国国家生物技术信息中心(NCBI)数据库对莲NnOMT、NnNMT基因家族进行鉴定,分析其理化性质及亚细胞定位。利用TBtools、MEME、MEGA 11.0、FigTree 1.4.4等工具对前期鉴定出的NnOMT、NnNMT基因的系统发育、序列特征、基因结构、功能注释、顺式作用元件等进行分析。结果:共鉴定出61个莲NnOMT、NnNMT基因,编码的氨基酸数目介于168~580 aa,等电点范围为4.76~9.16,相对分子质量为18699.52~64934.53 Da,大部分表现为酸性且多为亲水蛋白,含有10个保守基序;京都基因与基因组百科全书(KEGG)分析共富集到12个通路,包括新陈代谢、异喹啉生物碱生物合成、苯丙烷的生物合成等;基因本体(GO)富集结果可视化显示,61个莲NnOMT、NnNMT基因共注释到32个类别,其中包括16项分子功能,如谷氨酸合成酶还原型烟酰胺腺嘌呤二核苷酸(NADH)活性、外肽酶活性,以及16项生物过程,如四氯化碳代谢过程、厌氧四氯化碳代谢过程、对外源生物刺激的反应。在莲NnOMT、NnNMT基因的启动子区存在多种顺式作用元件,主要为启动子和增强子区响应元件、光响应和茉莉酸甲酯响应元件。结论:该研究基于莲的基因组数据对61个莲NnOMT、NnNMT基因进行了全面的生物信息学分析,为莲NnOMT、NnNMT基因家族的基因结构和功能研究奠定了基础,并为莲BIAs的生物合成途径解析提供了参考。Objective:The biosynthetic pathways of benzylisoquinoline alkaloids(BIAs)in Nelumbo nucifera are of great theoretical and economic value.In this paper,N.nucifera O-methyltransferase(NnOMT)and N.nucifera N-methyltransferase(NnNMT)gene families were identified and analyzed by bioinformatics in order to facilitate the biosynthetic pathway of BIAs in N.nucifera.Method:Based on the whole genome of N.nucifera,UniPort and National Center for Biotechnology Information(NCBI)databases were used to identify the NnOMT and NnNMT gene families of N.nucifera,and analyze their physicochemical properties and subcellular localization,then TBtools,MEME,MEGA 11.0,FigTree 1.4.4 and other tools were used to analyze the phylogeny,sequence characteristics,gene structure,functional annotation and cis-acting elements of NnOMT and NnNMT genes identified in the previous stage.Result:A total of 61 NnOMT and NnNMT genes were identified in this paper,the number of amino acids encoded by these genes ranged from 168 aa to 580 aa,the isoelectric point ranged from 4.76 to 9.16,and the relative molecular weight ranged from 18699.52 Da to 64934.53 Da,most of which showed acidic and mostly hydrophilic proteins.There were 10 conserved motifs,Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis enriched a total of 12 pathways,including metabolism,biosynthesis of phenylpropane and isoquinoline alkaloids,etc.And Visualization of Gene Ontology(GO)enrichment results showed that 61 NnOMT and NnNMT genes were annotated to 32 items,which included 16 molecular functions[such as reduced nicotinamide adenine dinucleotide(NADH)activity and exopeptidase activityJ and 16 biological processes(such as metabolic process of carbon tetrachloride,anaerobic carbon tetrachloride metabolic process and responses to exogenous biological stimuli).There were a variety of cis-acting elements in the promoter regions of NnOMT and NnNMT genes,mainly promoter and enhancer regions element,light responsive element and methyl jasmonate responsive element.Conclusion:In this study,a com

关 键 词：莲 氧甲基转移酶(OMT) 氮甲基转移酶(NMT) 生物信息学 功能分析 基因家族 异喹啉生物碱 

分 类 号：R22[医药卫生—中医基础理论] Q343.1[医药卫生—中医学] R28[生物学—遗传学] R931