GPS2对肝癌细胞系MHCC-97H增殖、迁移与侵袭的调控作用  

The role of GPS2 in regulating proliferation,migration and invasion of hepatocellular carcinoma cell line MHCC-97H

作　　者：刘爱霞 张浩 孙杰 杨丽华 赵晓涛[1] LIU Aixia;ZHANG Hao;SUN Jie;YANG Lihua;ZHAO Xiaotao(Department of Clinical Laboratory,Peking University People′s Hospital,Beijing 100044;Department of Clinical Laboratory,the Fifth Medical Centre of PLA General Hospital,Beijing 100039;Yanda Medical Research Institute,Hebei Yanda Hospital,Langfang 065201,China)

机构地区：[1]北京大学人民医院检验科,北京100044 [2]解放军总医院第五医学中心检验科,北京100039 [3]河北燕达医院燕达医学研究部,河北廊坊065201

出　　处：《基础医学与临床》2023年第12期1784-1791,共8页Basic and Clinical Medicine

基　　金：国家科技重大专项(2017ZX10302201)。

摘　　要：目的探索G蛋白通路抑制因子2(G protein pathway suppressor 2,GPS2)对肝癌细胞增殖、迁移与侵袭的调控作用及分子机制。方法在肝细胞癌(HCC)细胞系MHCC-97H中,使用定量PCR或Western blot检测HCC细胞中GPS2等的表达水平;在HCC细胞中进行转染,分别使用GPS2的表达载体、GPS2基因的shRNA慢病毒载体在HCC细胞中对GPS2进行过表达或者敲低;同时采用克隆形成实验、划痕实验以及Transwell实验等检测GPS2对HCC细胞增殖、迁移与侵袭的影响。结果在HCC细胞中,过表达GPS2能够抑制HCC细胞系MHCC-97H的增殖、迁移与侵袭,而敲低GPS2能够上调其增殖、迁移与侵袭(P<0.05)。进一步,在MHCC-97H细胞中过表达GPS2能够抑制EGFR、MMP1、MMP3以及MMP9等细胞增殖、迁移与侵袭相关因子的表达水平,而敲低GPS2的表达则能够上调这些因子的表达水平(P<0.05)。结论GPS2是新型HCC调控因子,能够抑制HCC细胞系的增殖、迁移与侵袭。Objective To explore the role of G protein pathway suppressor 2(GPS2)in regulating proliferation,migration and invasion in hepatocellular carcinoma cell line MHCC-97H.Methods The expression of GPS2 in hepatocellular carcinoma(HCC)cells were detected using quantitative PCR or Western blot;GPS2 was either over-expressed or knocked down in HCC cells using over-expression vectors or short hairpin RNAs(shRNAs)for GPS2,respectively.Clone formation assay,scratch assay and Transwell assay were used to detect the effects of GPS2 on proliferation,migration and invasion of HCC cells.Results In HCC cells,over-expression of GPS2 was found to inhibit the proliferation,migration and invasion of HCC cells MHCC-97H,while knockdown of GPS2 up-regulated proliferation,migration and invasion of MHCC-97H(P<0.05).The over-expression of GPS2 in MHCC-97H cells was found to inhibit the expression of cell proliferation,migration and invasion-related factors such as EGFR,MMP1,MMP3 and MMP9,while knockdown of GPS2 upregulated the expression of these factors(P<0.05).Conclusions GPS2 is a novel HCC regulatory factor that inhibits proliferation,migration and invasion of HCC cells.

关 键 词：肝细胞癌 G蛋白通路抑制因子2(GPS2) 细胞增殖 迁移与侵袭 

分 类 号：R394.3[医药卫生—医学遗传学]