响应面法优化紫茎泽兰中花色苷的提取工艺及其抗氧化活性研究  

Optimization of Ultrasonic-Assisted Double Enzymatic Extraction of Anthocyanins from Perilla Leaf and Study on Antioxidant Activity

作　　者：罗承梅 朱文婷 屠霜茹 李颖慧 周雪培 Luo Chengmei;Zhu Wenting;Tu Shuangru;Li Yinghui;Zhou Xuepei(Wuhan Technology and Business University,College of Environmental and Biological Engineering,Wuhan 430065,China)

机构地区：[1]武汉工商学院环境与生物工程学院,湖北武汉430065

出　　处：《山东化工》2023年第24期62-65,共4页Shandong Chemical Industry

基　　金：2022年湖北省大学生创新创业训练计划项目,项目名称《基于对环境有毒害性外来入侵植物资源的开发利用——响应面优化紫茎泽兰叶片提取花色苷的工艺研究及其抗氧化活性分析》,项目编号:S202213242004。

摘　　要：以紫茎泽兰叶片为研究对象,以花色苷提取率为考察指标,筛选出110℃烘干为干燥方式。利用超声辅助法提取紫茎泽兰叶片中的花色苷,研究不同超声时间、提取时间、乙醇体积分数、液料比对紫茎泽兰叶中花色苷提取的影响,通过单因素考察及响应面法确定紫茎泽兰叶片中花色苷提取的最佳提取工艺路线,并通过1,1-二苯基-3-三硝基苯肼自由基法、羟基自由基法评价紫茎泽兰叶片中花色苷提取物的体外抗氧化活性,为紫茎泽兰工业化发展提供技术路线。Taking the leaves of Eupatorium adenophorum as the research object,and taking the extraction rate of anthocyanins as the index,drying at 110℃was selected as the drying method.Ultrasonic assisted extraction of anthocyanins from Eupatorium adenophorum leaves was used to study the effects of different ultrasonic time,extraction time,ethanol concentration and liquid-material ratio on the extraction of anthocyanins from Eupatorium adenophorum leaves.The optimal extraction process route of anthocyanins from Eupatorium adenophorum leaves was determined by single factor investigation and response surface methodology Hydroxyl radical method was used to evaluate the in vitro antioxidant activity of anthocyanin extract from Eupatorium adenophorum leaves,which provided a technical route for the industrial development of Eupatorium adenophorum.

关 键 词：紫茎泽兰叶片 响应面优化 花色苷提取率 抗氧化活性 

分 类 号：TQ914.1[化学工程]