葡萄不同性别花器官发育机制初探  

Preliminary Study on the Mechanism of Flower Organ Development and Sex Formation in Different Grapes

作　　者：韩凯 罗尧幸 焦晓博 闫钊[1] Naomi Abe-Kanoh 马小河 纪薇[2,3] HAN Kai;LUO Yaoxing;JIAO Xiaobo;YAN Zhao;NAOMI Abe-Kanoh;MA Xiaohe;JI Wei(Department of Materials and Chemical Engineering,Taiyuan University,Taiyuan 030032,China;College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Faculty of Agriculture,Yamagata University,Yamagata 997-8555,Japan;Pomology Institute,Shanxi Agricultural University,Taigu,Shanxi 030815,China)

机构地区：[1]太原学院材料与化学工程系,太原030032 [2]山西农业大学园艺学院,山西太谷030801 [3]山形大学农学院,日本山形997-8555 [4]山西农业大学果树研究所,山西太谷030815

出　　处：《园艺学报》2023年第12期2551-2567,共17页Acta Horticulturae Sinica

基　　金：山西省科技成果转化引导专项项目(202204021301028);山西省专利转化专项计划项目(202301002);山西省高等学校优秀成果(科学技术)培育项目(2020KJ037);山西省现代农业产业技术体系建设专项(2023CYJSTX07-01)。

摘　　要：以‘北醇’葡萄两性花、‘1613C’葡萄雄花和‘520A’葡萄雌花为材料,测定始蕾期、大蕾期和盛花期花器官的激素含量并进行转录组测序。各激素在不同性别花中的积累量不同,同一性别花器官不同发育时期激素含量也有差异,在大蕾期雌花中IAA、ABA、MeJA含量最高。转录和代谢联合分析共获得31965个表达基因,其中与花发育直接相关的基因1535个,部分基因持续上调或下调,持续上调的基因主要参与苯丙烷合成、植物激素信号转导等通路,持续下调基因主要参与淀粉和糖代谢、氨基酸合成等通路。AP1、SEP1、AG2、SOC1等MADS-box基因在不同花器官和不同发育时期差异表达。MADS-box基因、开花诱导整合子基因及解毒和应激相关基因的差异表达对花性别分化具有重要作用。In the current study,the flowers of three grape varieties‘Beichun'(hermaphrodite),‘1613C'(male)and‘520A'(female)were chosen.Hormone determination and transcriptome sequencing were performed at the early bud stage,large bud stage and blooming stage.The accumulation of various plant hormones in flowers of different genders was different,and so was that in flowers of the same gender at different developmental stages.The contents of IAA,ABA and MeJA were the highest in the large bud stage of female flowers.A total of 31965 expressed genes were obtained,and 1535 genes were directly related to flower development and were mainly involved in phenylpropane synthesis and plant hormone signal transduction pathways.Some genes are continuously up-regulated or down-regulated at different developmental stages.The genes that are continuously up-regulated are mainly involved in Phenylpropane synthesis,plant hormone signal transduction and other pathways.The genes that are continuously down-regulated are mainly involved in starch and sugar metabolism,and amino acid synthesis.MADS-box genes,such as AP1,SEP1,AG2 and SOC1 were significantly differently expressed in different floral organs at developmental stages.During flower sex differentiation,the differential expression of the MADS-box gene,the flower-induced integron gene,and the detoxification and stress-related genes play important roles.

关 键 词：葡萄 花器官 RNA-SEQ 内源激素 花性别分化 

分 类 号：S663.1[农业科学—果树学]