煅烧对僧帽牡蛎壳的形态与抗氧化及凝血活性的影响  

Effects of calcination on the morphology,antioxidant and coagulant activities in vitro of the shells of Saccostrea cucullata

作　　者：SUWANNASINGHA Nattasak 袁炎炎 李昕 KLAYPRADIT Wanwimol 刘红兵[1,3] SUWANNASINGHA Nattasak;YUAN Yanyan;LI Xin;KLAYPRADIT Wanwimol;LIU Hongbing(Key Laboratory of Marine Drugs,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Faculty of Fisheries,Kasetsart University,Bangkok 10900,Thailand;NMPA Key Laboratory for Quality Research and Evaluation of Marine Traditional Chinese Medicine,Qingdao 266000,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]泰国农林大学水产学院,泰国曼谷10900 [3]国家药品监督管理局海洋中药质量研究与评价重点实验室,山东青岛266000

出　　处：《中国海洋药物》2023年第6期29-34,共6页Chinese Journal of Marine Drugs

基　　金：NMPA海洋中药质量研究与评价重点实验室自主研究课题(HYZY-ZZKT-2022002)资助。

摘　　要：目的研究煅烧对僧帽牡蛎壳的形态、体外抗氧化和凝血活性的影响。方法采用扫描电子显微镜-能谱仪(SEM-EDS)观察表面形态及元素变化;利用DPPH法及FRAP法评价抗氧化活性;使用凝血分析仪检测对凝血因子APTT、PT和TT的影响。结果僧帽牡蛎壳经煅烧后,表面性状发生变化,其清除DPPH自由基和总抗氧化能力增强,且随着煅烧温度的升高而增加,并显示一定的浓度依赖性;煅烧后的牡蛎壳能显著缩短凝血酶原时间PT,提示具有促凝血作用。结论僧帽牡蛎壳煅烧后抗氧化活性和止血作用增强,研究为僧帽牡蛎的综合开发利用提供基础数据。Objective To reveal effects of calcination on the morphology,antioxidant and coagulant activities in vitro of the shells of oyster Saccostrea cucullata(OS).Methods Scanning electron microscopy and energy dispersive spectroscopy(SEM-EDS)were used for the morphological observation.The antioxidant activities were evaluated by DPPH method and FRAP method in vitro.Activated partial thromboplastin time(APTT),prothrombin time(PT)and thrombin time(TT)were measured by a coagulation analyzer.Results The surface granule of calcined OS was rougher than that of non-calcined OS,and their element contents were related to the calcination temperatures.The antioxidant activities of calcined samples were increased,which positively correlated with temperature and in a dose-dependent manner.Meanwhile,the calcined OS showed significantly procoagulant effect by shortening PT.Conclusion Calcined shells of OS exhibited antioxidant and procoagulant activities in vitro,which provided basic data for the comprehensive development and utilization.

关 键 词：僧帽牡蛎 贝壳 煅烧 扫描电子显微镜-能谱仪 抗氧化活性 凝血作用 

分 类 号：R37-1155[医药卫生—病原生物学]