坛紫菜丝状体辐射诱变后的筛选与培养  

Screening and Culture of Free-Living Conchocelis in Neoporphyra haitanensis After Radiation Mutagenesis

作　　者：许万涛 秦欣 陈海敏[1] 骆其君[1] XU Wantao;QIN Xin;CHEN Haimin;LUO Qijun(School of Marine Science,Ningbo University,Ningbo,Zhejiang 315211)

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《核农学报》2023年第12期2327-2333,共7页Journal of Nuclear Agricultural Sciences

基　　金：宁波市重大科技项目(2021ZDYF020081);现代农业产业技术体系专项资金(CARS-50);浙江省重大科技专项(2021C02069-9)。

摘　　要：为建立合适的筛选坛紫菜丝状体突变体的方法,将经^(60)Co-γ射线诱变的丝状体分别接种到海水和扇贝壳中,观测丝状体的突变率。结果表明,丝状体经γ射线辐射后,仍具有钻入扇贝壳的能力,在扇贝壳上发生了丰富的颜色突变,在1000 Gy辐射剂量下丝状体突变率最高,各不同诱变梯度下,在自由丝状体阶段的突变率均低于在扇贝壳丝状体的阶段。此外,为探究突变体的适宜生长温度和培养介质,将^(60)Co-γ射线辐射诱变得到的粉色和绿色突变体为材料,选取扇贝壳、琼脂培养基和海水等基质,设置4个温度梯度(14、17、20、23℃),观测突变藻丝细胞的长度、宽度及分枝数量变化。结果表明,粉色和绿色突变体在23℃的培养温度和扇贝壳基质中,突变性状较稳定,生长状况较佳,粉色突变体藻丝细胞的长度、宽度及分枝数量分别为190.35μm、12.23μm、10.00个,藻丝细胞的宽度和分枝数量的增长率约为19.43%和28.21%;绿色突变体藻丝细胞的长度、宽度及分枝数量分别为115.89μm、11.26μm、3.80个,藻丝细胞的宽度增长率约为9.96%。本研究结果为坛紫菜自由丝状体突变体的筛选提供了新的方法,为探索突变体适宜生长发育的培养温度及基质提供了参考。To establish an appropriate method for screening free-living conchocelis mutants of Neoporphyra haitanensis,the conchocelis were exposed to ^(60)Co-γ-ray irradiation.Subsequently,the irradiated conchocelis were separately cultured in seawater and on scallop shells to observe the mutation rate of the conchocelis.The results indicated that,after exposure toγ-ray irradiation,conchocelis retained the ability to penetrate scallop shells,and showed rich color mutations.The highest mutation rate was observed at a radiation dose of 1000 Gy,with the mutation rates of free-living conchocelis being consistently lower than those of shell-borne conchocelis under different radiation intensities.Furthermore,to investigate the optimal growth temperature and culture medium for mutants,pink and green mutants induced by ^(60)Co-γ-ray radiation were cultured on scallop shells,agar medium,and in seawater.Four temperature gradients(14,17,20,23℃)were set,and the changes in the length,width,and branching of mutant conchocelis cells were observed.The results revealed that the pink and green mutants exhibited relatively stable mutation and the growths were optimum when grown at a temperature of 23℃on scallop shell substrates.In the case of the pink mutant,the conchoceli had a length of 190.35μm,a width of 12.23μm,and branches numbers of 10.00.The growth rates for width and branching numbers were approximately 19.43%and 28.21%,respectively.For the green mutant,the conchoceli’s length,width,and branching numbers were 115.89μm,11.26μm,and 3.80,respectively,with a width growth rate of approximately 9.96%.Hence,the results provided a new method for screening free-living conchocelis mutants of N.haitanensis,and could be beneficial for exploring the optimal cultivation temperature and substrates for the growth and development of mutants.

关 键 词：坛紫菜 ^(60)Co-γ射线 丝状体 诱变 突变体 

分 类 号：S968.431[农业科学—水产养殖]