磁性碳纳米管的制备及其在肿瘤细胞光热疗与磁共振成像中的应用  

Preparation of magnetic carbon nanotubes and their application in tumor cell photothermal therapy and magnetic resonance imaging

作　　者：王晓驰 景亚 张光辉[1] 左振宇[1] WANG Xiaochi;JING Ya;ZHANG Guanghui;ZUO Zhenyu(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院,咸阳712046

出　　处：《复合材料学报》2023年第12期6545-6553,共9页Acta Materiae Compositae Sinica

基　　金：陕西省重点研发计划一般项目(2021SF-366);陕西省教育厅重点项目(20JS033)。

摘　　要：肿瘤是目前最主要的致死原因之一,实现对肿瘤的精准和非侵入性高效诊疗具有重要意义。以具有极高长径比、易于穿透细胞膜并具有优异生物相容性的碳纳米管(CNTs)作为载体,以乙酰丙酮铁为铁源,通过溶剂热法在其表面原位生长具有超顺磁特性的四氧化三铁纳米粒子(Fe_(3)O_(4)NPs),制备了具有优异水分散稳定性的磁性碳纳米管复合纳米材料。结果表明该磁性碳纳米管具有较高的近红外光热转换性能,在50μg·mL^(-1)浓度下808 nm激光照射10 min即可升温至48.6℃,且具有良好的光热稳定性。细胞及成像实验结果表明该复合纳米材料具有较好的生物相容性并对人宫颈癌细胞(HeLa)具有优异的光热杀伤效果,在体外模拟肿瘤微环境中磁共振成像(MRI)T_(2)弛豫率r_(2)可达215.61 mmol^(-1)·L·s^(-1),表明制备的磁性碳纳米管具有出色的生物安全性、磁性和光热特性,有望用于磁靶向的肿瘤光热疗与磁共振成像的一体化诊疗。Tumors are one of the leading causes of death in the world,and achieving precise and non-invasive efficient diagnosis and treatment of tumors is of great significance.We used carbon nanotubes(CNTs)with extremely high aspect ratio,easy to penetrate cell membrane and excellent biocompatibility as carriers,and acetylacetone iron as iron source,to synthesize magnetic carbon nanotube composite nanomaterials with excellent water dispersion stability by in situ growing superparamagnetic ferric oxide nanoparticles(Fe_(3)O_(4) NPs)on their surface through solvothermal method.The results showed that the magnetic carbon nanotubes had high near-infrared photothermal conversion performance,and could reach 48.6℃in 10 min under 808 nm laser irradiation at a concentration of 50μg·mL^(−1),and had good photothermal stability.Cell and imaging experiments showed that the composite nanomaterials had good biocompatibility and excellent photothermal killing effect on human cervical cancer cells(HeLa).In vitro simulated tumor microenvironment,the magnetic resonance imaging(MRI)T_(2) relaxation rate r_(2) of the magnetic carbon nanotubes was up to 215.61 mmol^(−1)·L·s^(−1),indicating that the prepared magnetic carbon nanotubes had outstanding biosafety,magnetism and photothermal properties,and were expected to be applied to the integration of magnetic targeted tumor photothermal therapy and magnetic resonance imaging.

关 键 词：碳纳米管 磁性Fe_(3)O_(4)纳米粒子 光热治疗 磁共振成像 肿瘤细胞 

分 类 号：R730.5[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]