蛋白质二硫键异构酶结构与相关疾病研究进展  

Research progress of structures and related diseases of protein disulfide isomerase

作　　者：葛璟燕 檀维 洪大伟 张蓓 GE Jingyan;TAN Wei;HONG Dawei;ZHANG Bei(College of Biological Engineering,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《浙江工业大学学报》2024年第1期112-118,共7页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(21877100)。

摘　　要：蛋白质二硫键异构酶(Protein disulfide isomerase,PDI)属于硫氧还蛋白家族,其分子质量为57 kD,主要存在于内质网中,且结构中有两个活性位点,在蛋白质折叠过程中催化二硫键的裂解、形成和重排。PDI活性异常会导致未折叠或错误蛋白的积累,引起内质网应激(ERs),从而导致未折叠蛋白反应(UPR)。研究表明:PDI与癌症、神经紊乱等疾病显著相关,PDI的表达水平升高与多种癌症的发生、发展相关。通过对PDI的蛋白结构、功能、活性检测及相关疾病进行综述,以期为PDI及其病理研究提供新思路。Protein disulfide isomerase(PDI),with a molecular weight of 57 kD,belongs to thioredoxin family and has two active sites in its structure.It catalyzes the cleavage,formation,and rearrangement of disulfide bondin the process of protein folding.Abnormal PDI activity can lead to the accumulation of unfolded or misfolded proteins,causing endoplasmic reticulum stresses(ERs),which can result in an unfolded protein response(UPR).Evidence suggested that PDI is significantly associated with diseases,including cancers and neurological disorder,and that elevated levels of PDI expression are associated with the development of various cancers.Herein,this review provides recentadvance on the structure,function,activity detection and relative diseases of PDI.

关 键 词：蛋白质二硫键异构酶 结构 功能 活性检测 癌症 

分 类 号：Q7[生物学—分子生物学] R73[医药卫生—肿瘤]