基于V1区种子点应用静息态功能磁共振的功能连接技术分析正常眼压性青光眼患者脑部功能连接的变化  

Study of brain regions of normal tension glaucoma patients by functional connectivity technology in resting-state functional magnetic resonance imaging based on V1 region seed point

作　　者：臧苗苗 范彩媚 姜建[2] 邵毅[1] 王丽君 曾璐瑶 易澄 武天硕 李汉林[1] ZANG Miaomiao;FAN Caimei;JIANG Jian;SHAO Yi;WANG Lijun;ZENG Luyao;YI Cheng(WU Tianshuo 1,LI Hanlin 11.Department of Ophthalmology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China;Department of Imaging,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学第一附属医院眼科,江西省南昌市330006 [2]南昌大学第一附属医院影像科,江西省南昌市330006

出　　处：《眼科新进展》2024年第2期112-117,共6页Recent Advances in Ophthalmology

基　　金：江西省应用研究培育计划(编号:20212BAG70032);江西省重点研发计划(编号:20202BBGL73019);江西省卫计委科技计划项目(编号:202130275)。

摘　　要：目的基于V1区种子点(ROI)应用静息态功能磁共振的功能连接技术研究正常眼压性青光眼(NTG)患者和健康志愿者的脑部功能连接变化,旨在探究NTG患者的发病机制及早期诊断方法。方法收集符合纳入标准的NTG患者14例(NTG组)及健康对照者14例(HCs组),收集受试者的临床数据信息后对两组受试者行静息态功能性磁共振成像扫描。通过软件对磁共振数据进行预处理,以双侧V1区作为ROI,分别计算其与全脑体素时间序列的相关性并比较组间静息态功能连接的差异得到V1区ROI和全脑的功能连接值。采用Pearson相关分析探讨NTG组患者与V1区功能连接显著差异脑区功能连接值与临床变量之间的相关性。结果与HCs组受试者相比,NTG组患者年龄、性别、体重、杯盘比、24 h平均眼压差异均无统计学意义(均为P>0.05),两组患者间左、右眼最佳矫正视力(BCVA)及视盘周围视网膜神经纤维层厚度(RNFLT)差异均有统计学意义(均为P<0.05)。Pearson相关性分析结果显示,NTG组患者与V1区异常功能连接脑区功能连接值均与RNFLT具有相关性(P<0.05)。ROI1-左侧额上回、ROI1-右侧额上回、ROI2-左侧扣带回和ROI2-右侧额中回与RNFLT均呈显著正相关(均为P<0.05)。与HCs组受试者相比,NTG组患者与右侧ROI功能连接减低的脑区为左侧额上回及右侧额上回;与左侧ROI功能连接减低的脑区为左侧扣带回和右侧额中回。结论相较于健康人,NTG患者某些特定大脑区域与V1区的功能连接有显著改变,包括双侧额上回、左侧扣带回、右侧额中回。大脑功能活动的变化区域可能由NTG引起的视觉功能障碍导致视觉和认知情绪处理脑区的功能损伤,这可能是NTG患者潜在神经病理机制之一。Objective To study the brain functional connectivity(FC)changes in patients with normal tension glaucoma(NTG)and healthy volunteers using FC technique of resting-state functional magnetic resonance imaging(rs-fMRI)based on V1 region seed point(ROI),so as to explore the pathogenesis and early diagnosis of NTG.Methods Fourteen NTG patients(NTG group)who met the inclusion criteria and 14 healthy controls(HCs group)were enrolled.The clinical data of all subjects were collected,and rs-fMRI was performed in both groups.The magnetic resonance data was pre-processed by software,and bilateral A1 regions were taken as the ROI to analyze their correction with the whole brain voxel time series and obtain the FC value between the ROI and the whole brain by comparison of FC values in resting state between the groups.Pearson correlation analysis was used to explore the relationship between FC value in the brain regions with significant differences with the ROI and clinical variables in the NTG group.Results Compared with the subjects in the HCs group,there were no statistically significant differences in age,gender,body weight,cup-disc ratio and 24 h mean intraocular pressure of patients in the NTG group(all P>0.05),and there were statistically significant differences in the best corrected visual acuity(BCVA)of both eyes and peripapillary retinal nerve fiber layer thickness(RNFLT)(all P<0.05).The Pearson correlation analysis showed that FC value of the brain regions with abnormal FC to V1 region were correlated with RNFLT in the NTG group(P<0.05).ROI1-left superior frontal gyrus,ROI1-right superior frontal gyrus,ROI2-left cingulate gyrus and ROI2-right middle frontal gyrus were significantly positively correlated with RNFLT(all P<0.05).Compared with the HCs group,the brain regions with reduced FC to the right ROI in the NTG group were the left superior frontal gyrus and right superior frontal gyrus;the brain regions with reduced FC to the left ROI were the left cingulate gyrus and right middle frontal gyrus.Conclusion Compared

关 键 词：正常眼压性青光眼 静息态功能磁共振 功能连接技术 V1区种子点 

分 类 号：R775[医药卫生—眼科]