川赤芍总苷提取工艺优化、组成和抗氧化活性分析  

Optimization of extraction of total glycosides from Paeonia veitchii and analysis of its composition and antioxidant activity

作　　者：金鑫[1] 马小龙 李萍[1] 熊川[1] 黄文丽[1] 朱宇[1] JIN Xin;MA Xiaolong;LI Ping;XIONG Chuan;HUANG Wenli;ZHU Yu(Biotechnology and Nuclear Technology Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610061;Sichuan Aba County Productivity Promotion Center,Aba Prefecture 624600)

机构地区：[1]四川省农业科学院生物技术核技术研究所,成都610061 [2]四川省阿坝县生产力促进中心,阿坝州624600

出　　处：《中国食品添加剂》2024年第1期109-118,共10页China Food Additives

基　　金：四川省科技支撑项目(2020ZHFP0195)。

摘　　要：探究超声波辅助乙醇提取川赤芍总苷,通过单因素和响应面优化提取工艺,采用高效液相色谱(HPLC)法检测总苷组成,并通过体外抗氧化试验评价总苷的抗氧化活性。结果表明:最佳提取工艺为超声功率175 W、超声时间42 min、乙醇浓度70%、液料倍数30(mL/g),此工艺条件下提取的川赤芍总苷得率为(5.86±0.32)%。HPLC分析总苷共检测到21个特征峰,其中没食子酸0.63 mg/g、氧化芍药苷11.28 mg/g、儿茶素18.64 mg/g、芍药内酯苷5.82 mg/g、芍药苷54.82 mg/g、苯甲酰芍药苷15.74 mg/g、丹皮酚原苷0.98 mg/g,共占总质量的95.35%。总苷清除DPPH·、ABTS+·和·OH-的IC50值分别为0.78、1.13、0.98 mg/mL,总苷的抗氧化活性强弱与浓度呈现较好的正相关。The study investigated ultrasonic-assisted ethanol extraction of total glycosides of Paeonia veitchii.The extraction process was optimized through single factor and response surface methodology;The composition of total glycosides was detected by high performance liquid chromatography(HPLC)and antioxidant activity of total glycosides was evaluated through in vitro antioxidant test.The results showed that the optimum extraction process was as follow:ultrasonic power 175 W,ultrasonic time 42 min,ethanol concentration 70%,liquid-solid ratio 30(mL/g).Under the above conditions,the yield of total glycosides of Paeonia veitchii was(5.86±0.32)%.A total of 21 characteristic peaks were detected in the HPLC analysis of total glycosides,including gallic acid 0.63 mg/g,oxidized paeoniflorin 11.28 mg/g,catechin 18.64 mg/g,paeoniflorin 5.82 mg/g,paeoniflorin 54.82 mg/g,benzoyl paeoniflorin 15.74 mg/g,and paeoniflorin 0.98 mg/g,accounting for 95.35%of the total mass.The IC50 values of total glycosides for scavenging DPPH·,ABTS+·and·OH-free radicals were 0.78,1.13 and 0.98 mg/mL,respectively.The antioxidant activity of total glycosides was positively correlated with their concentration.

关 键 词：川赤芍 总苷 响应面 超声波 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学] R730.5[轻工技术与工程—食品科学与工程]