6株H9N2亚型禽流感病毒分子特征及遗传演化分析  

Molecular characterization and genetic evolution of six H9N2 subtype avian influenza virus strains

作　　者：邢燕茹 祝宇翔 范春燕 于海[2] 汪秀会 XING Yanru;ZHU Yuxiang;FAN Chunyan;YU Hai;WANG Xiuhui(School of Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,China;Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Shanghai 200241,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《畜牧与兽医》2024年第2期89-97,共9页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金面上项目(31970175);中国农业科学院创新工程“猪病毒性繁殖障碍疫病防控技术团队”项目;河北省邯郸市科学技术研究与发展计划项目(19422011008-45);河北省自然科学基金资助项目(C2020402010)。

摘　　要：为了解H9N2亚型禽流感病毒(avian influenza virus,AIV)的分子变化特征及其跨物种传播的可能性,对实验室保存的6株H9N2亚型AIV的8个基因片段进行了序列测定及遗传演化分析,并且对6株病毒与选取的代表毒株进行了HA和NA同源性及关键氨基酸位点分析。结果显示,分离的6株病毒均为欧亚系,其中3株为G57基因型;4株病毒的HA蛋白发生了Q226L突变,2株病毒的HA发生了A190V突变。HA和NA均有增加或缺失的潜在糖基化位点。6株病毒的NA茎部均有62~64位的缺失,红细胞结合位点431~433较为保守,而368和369位变化较大。综上,6株病毒为低致病性AIV,HA和NA均含有哺乳动物适应性突变,增大了其跨宿主传播的风险。研究结果为我国H9N2亚型AIV的进化提供了参考依据,为其综合防控提供了理论指导。In order to understand the molecular characteristics of H9N2 subtype avian influenza virus(AIV)and the possibility of cross-species transmission,8 gene fragments of 6 strains of AIV preserved in the laboratory were sequenced,and their genetic evolution was analyzed.The homology of HA and NA and the key amino acid sites between 6 strains and selected representative strains were analyzed.The results showed that all the 6 strains belonged to the Eurasian lineage,and three of them were of the G57 genotype.4 strains had Q226L mutation in HA,and 2 strains had A190V mutation in HA.Both HA and NA had increased or decreased potential glycosylation sites.62-64 sites were absent in the NA stalk of all the 6 strains,and the hemadsorbing sites 431-433 were conserved,while the 368 and 369 sites had significant changes.In conclusion,the 6 strains were low pathogenic avian influenza viruses with mammalian adaptive mutations in both HA and NA,which increased the risk of cross-species transmission.This study supplies reference for the evolution of H9N2 subtype AIV in China,and provides theoretical guidance for comprehensive prevention and control of the virus.

关 键 词：禽流感病毒 H9N2亚型 分子特征 遗传进化 

分 类 号：S852[农业科学—基础兽医学]