复合功能菌群对于石油烃降解机理及菌群结构研究  

Degradation mechanisms of functional microbial communities on petroleum hydrocarbons and microbial community structures study

作　　者：杨勇 杨庭 李凤祥[3] 刘忠源 YANG Yong;YANG Ting;LI Feng-xiang;LIU Zhong-yuan(School of Chemical Engineering,Tianjin University,Tianjin 300072,China;China Offshore Environmental Service Ltd.,Tianjin 300457,China;College of Environmental Science and Engineering,Nankai University,Tianjin 300350,China)

机构地区：[1]天津大学化工学院,天津300072 [2]中海石油环保服务(天津)有限公司,天津300457 [3]南开大学环境科学与工程学院,天津300350

出　　处：《应用化工》2023年第12期3227-3231,3251,共6页Applied Chemical Industry

基　　金：国家重点研发计划(2018YFC1801905)。

摘　　要：开展了复合功能菌群降解含油固废实验,通过分析二十八烷的降解情况,研究了复合功能菌群对石油烃的降解机理,对降解前后的微生物群落结构及群落演变进行高通量测序分析。结果表明,复合菌群对于石油烃的降解率为81.30%,石油烃在烷烃单加氧酶的作用下被氧化为相应的伯醇,伯醇进一步氧化生成酸后进行β-氧化,每次脱掉两个碳生成乙酰辅酶A进入三羧酸循环,将烷烃完全分解成CO 2和H 2O。降解过程中,一些兼性好氧菌会逐渐成为优势菌。功能微生物菌群中含有丰富的代谢路径,使得微生物菌群对各种复杂有机物具有高效的降解效果。该研究为复合功能菌群应用提供了理论基础和支撑。Through the experiment of oily solid waste treatment with complex functional bacteria,the degradation mechanism of complex functional bacteria on petroleum hydrocarbons(PHs)was studied by analyzing the degradation of octadecane.The microbial community structure and community evolution of oily solid waste before and after degradation were analyzed by illumina MiSeq high-throughput sequencing.The results showed that the degradation rate of PHs by complex functional bacteria was 81.30%,and the PHs were oxidized to the corresponding primary alcohols by terminal oxidation pathway under the action of alkane monooxygenase,and the primary alcohols were further oxidized to generate acids and then carried outβ-oxidation,each time two carbons were removed to generate acetyl-CoA into the tricarboxylic acid cycle,and the alkanes were gradually completely decomposed into CO 2 and H 2O.During the degradation process,some fastitutogenic aerobic bacteria gradually replaced obligate aerobic bacteria as dominant bacteria.The functional microbial communities contained abundant metabolic pathways,and these rich metabolic pathways made the microbial communities had efficient degradation effects on various complex organics.This study provides theoretical basis and support for the application of complex functional bacteria.

关 键 词：含油固废 石油烃降解 降解机理 群落结构演变 代谢途径 

分 类 号：X172[环境科学与工程—环境科学] X741