温胃阳汤对功能性消化不良大鼠肥大细胞活化及SCF/c-Kit信号通路的影响  

Effect of Wenweiyang decoction on mast cell activation and SCF/c-Kit signaling pathway in rats with functional dyspepsia

作　　者：税典奎 黎舒婷 黄慧花 龙海华[2] 杨健[2] 罗诗雨 覃凌娜 SHUI Diankui;LI Shuting;HUANG Huihua;LONG Haihua;YANG Jian;LUO Shiyu;QIN Lingna(Department of Gastroenterology,Liuzhou Traditional Chinese Medical Hospital(Liujcouh Si Ywcuengh Yihyen),Liuzhou 545001,China;Department of Gastroenterology,Liuzhou Municipal Liutie Central Hospital,Liuzhou 545007,China)

机构地区：[1]柳州市中医医院(柳州市壮医医院),广西柳州545001 [2]柳州市柳铁中心医院,广西柳州545007

出　　处：《中国病理生理杂志》2024年第1期74-80,共7页Chinese Journal of Pathophysiology

基　　金：广西自然科学基金资助项目(No.2020GXNSFAA259083);广西中医药适宜技术开发与推广项目(No.GZSY20-61)。

摘　　要：目的:基于肥大细胞活化及干细胞因子(stem cell factor,SCF)/受体酪氨酸激酶c-Kit信号通路探讨温胃阳汤治疗大鼠功能性消化不良的作用机制。方法:将60只SD大鼠随机分为空白组,模型组,雷尼替丁组及温胃阳汤低、中、高剂量组,每组10只。空白组大鼠不予造模,其他各组采用夹尾刺激加不规则喂养复合番泻叶法建立大鼠功能性消化不良模型,模型建立后,空白组及模型组灌胃给予生理盐水,温胃阳汤低、中、高剂量组和雷尼替丁组则分别用温胃阳汤(0.743 g/mL、1.485 g/mL和2.970 g/mL)及盐酸雷尼替丁胶囊(3 g/L)灌胃。治疗结束后,以碳墨推进法测定小肠推进率;采用甲苯胺蓝染色观察大鼠十二指肠组织肥大细胞并计数;ELISA测定大鼠十二指肠中肥大细胞类胰蛋白酶(mast cell tryptase,MCT)和组胺(histamine,HA)的含量;RT-qPCR检测十二指肠中SCF和c-Kit mRNA的表达;Western blot和免疫组化检测十二指肠中SCF和c-Kit蛋白的表达水平。结果:与模型组相比,温胃阳汤治疗显著提高大鼠的小肠推进率(P<0.05);ELISA结果显示,温胃阳汤治疗可减少大鼠十二指肠黏膜组织肥大细胞数量及MCT和HA含量(P<0.05);Western blot和免疫组化结果表明,温胃阳汤治疗可上调大鼠十二指肠组织c-Kit和SCF蛋白的表达水平(P<0.05),增加SCF和c-Kit阳性细胞数(P<0.05);RT-qPCR结果显示,WWYD治疗可上调大鼠十二指肠组织c-Kit和SCF mRNA的表达(P<0.01)。而且,小肠推进率分别与MCT和HA含量呈负相关,与SCF和c-Kit的表达呈正相关。结论:温胃阳汤能促进大鼠十二指肠动力,其作用机制可能与抑制大鼠十二指肠MCT和HA的生成,及激活SCF/c-Kit信号通路有关。AIM:To investigate the mechanism of action of Wenweiyang decoction(WWYD)in treating functional dyspepsia in rats based on mast cell activation and stem cell factor(SCF)/receptor tyrosine kinase c-Kit signaling pathway.METHODS:Sixty SD rats were randomly divided into control group,model group,ranitidine hydrochloride capsule group,and low-,medium-and high-dose WWYD groups,with 10 rats in each group.The rat model of functional dyspepsia was established by tail clamping and irregular feeding compound senna method.After modeling,the rats in control group and model group were given normal saline,while those in low-,medium-and high-dose(0.743 g/mL,1.485 g/mL and 2.970 g/mL)WWYD groups and ranitidine hydrochloride capsule(3 g/L)group were treated with corresponding drugs by intragastric administration.After treatment,the propulsion rate of the small intestine was measured by the carbon ink propulsion method.Rat duodenal mast cells were observed and counted by toluidine blue staining.ELISA was used for determination of mast cell tryptase(MCT)and histamine(HA)content in rat duodenum.The mRNA levels of SCF and cKit in duodenum were detected by RT-qPCR.Western blot and immunohistochemistry were employed to determine the expression levels of SCF and c-Kit in the duodenum.RESULTS:Compared with model group,WWYD treatment significantly increased the propulsion rate of the small intestine in rats(P<0.05).ELISA results showed that WWYD reduced the number of mast cells and the content of MCT and HA in the duodenal mucosa tissue of rats(P<0.05).Western blot and immunohistochemistry results suggested that WWYD up-regulated the protein expression levels of c-Kit and SCF in the duodenal tissue of rats(P<0.05),and increased the numbers of SCF and c-Kit positive cells.RT-qPCR results indicated that WWYD up-regulated the mRNA expression of c-Kit and SCF in the duodenum of rats(P<0.05).Moreover,the small intestinal propulsion rate was negatively correlated with MCT and HA content,and positively correlated with the expression of SCF and c-

关 键 词：功能性消化不良 温胃阳汤 肥大细胞 SCF/c-Kit信号通路 十二指肠 

分 类 号：R57[医药卫生—消化系统] R363.2[医药卫生—内科学]