北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析  

Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs

作　　者：牛乃琪 赵润泽 宗文成 刘先策 刘海 石国华 井西涛 张龙超 NIU Naiqi;ZHAO Runze;ZONG Wencheng;LIU Xiance;LIU Hai;SHI Guohua;JING Xitao;ZHANG Longchao(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Beijing Heiliu Stockbreeding Technology Co.Ltd.,Beijing 102211,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]北京黑六牧业科技有限公司,北京102211

出　　处：《畜牧兽医学报》2024年第1期79-86,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划项目(2021YFD1301101);国家生猪产业技术体系(CARS-35);中国农业科学院科技创新工程(ASTIP-IAS02)。

摘　　要：旨在开展GREB 1L和MIB 1基因多态性与肋骨数和胴体性状(胴体长、胴体直长、胴体斜长及胴体重)关联分析,筛选关键功能位点。本研究选取屠宰日龄为215天的317头健康北京黑猪,并提取耳组织DNA,对GREB 1L和MIB 1基因进行引物设计、并利用聚合酶链式反应(PCR)技术和Sanger测序技术等对SNPs进行基因分型,计算变异位点基因型频率以及等位基因频率分布,并与肋骨数和胴体性状进行关联分析。试验共筛选到6个SNPs,包括GREB 1L基因的3个同义突变、MIB 1基因的2个同义突变和1个内含子区的可变剪切突变。将6个SNPs分别对肋骨数和胴体性状使用Duncan’s多重检验统计分析发现,GREB 1L基因上的3个同义突变6:106574972 G>A、6:106648540 C>T、6:106648558 A>G与性状关联均不显著(P>0.05);MIB 1基因上的1个同义突变6:106936590 C>T与胴体长显著相关(P<0.05);而内含子15~16的6:107016475 A>G可变剪切突变与肋骨数显著相关(P<0.05);第三个同义突变6:107028660 G>A与肋骨数和胴体性状均无显著差异(P>0.05)。结果显示,显著的2个位点可作为北京黑猪肋骨数及胴体长变异的候选基因功能位点。The study aimed to conduct an association analysis of GREB 1L and MIB 1 gene polymorphisms with rib number and carcass traits(carcass length,straight length,diagonal length and carcass weight),in order to screen for key functional loci.In this study,DNA was extracted from ear tissue of 317 healthy Beijing black pigs at the age of 215 days.The primers were designed for GREB 1L and MIB 1.The exon regions of two genes were genotyped by PCR and Sanger sequencing.The distribution of genotype frequency and allele frequency of the mutated loci was calculated,and their association analysis with rib number and carcass traits was performed.A total of 6 SNPs were detected in two genes,including 3 synonymous mutations in GREB 1L,2 synonymous mutations in MIB 1,one splice donor 5th base variant-intron variant in MIB 1.Statistical analysis using Duncan’s multiple test revealed that 3 synonymous mutations(6:106574972 G>A,6:106648540 C>T,6:106648558 A>G)in GREB 1L and one synonymous mutation(6:107028660 G>A)in MIB 1 were no significant association with traits(P>0.05).A synonymous mutation in MIB 1(6:106936590 C>T)was significant association with carcass length and one splice donor 5th base variant-intron variant in MIB 1(6:107016475 A>G)was significant association with the number of rib(P<0.05).In conclusion,only two of six SNPs were significant differences in traits.A synonymous mutation of MIB 1(6:106936590 C>T)with carcass length and one splice donor 5th base variant-intron variant of MIB 1(6:107016475 A>G)with the number of rib,respectively.the above two significant SNPs could be used as candidate functional loci for the variation of rib number and carcass length of Beijing black pigs.

关 键 词：北京黑猪 GREB 1L MIB 1 肋骨数 胴体性状 

分 类 号：S828.2[农业科学—畜牧学]