缺氧预处理星形胶质细胞外泌体在SD大鼠脊髓神经元氧糖剥夺损伤的保护作用  

Protective effect of hypoxia preconditioned astrocytes exosomes on spinal neurons in SD rats with oxygen glucose deprivation injury

作　　者：朱玉堡 巢宋杰 高诗仑 谷天祥[1] 师恩祎[1] ZHU Yu-bao;CHAO Song-jie;GAO Shi-lun;GU Tian-xiang;SHI En-yi(Department of Cardiac Surgery,First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院心脏外科,辽宁沈阳110001

出　　处：《解剖科学进展》2023年第5期548-551,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81870963)。

摘　　要：目的探讨缺氧预处理星形胶质细胞外泌体对脊髓神经元氧糖剥夺损伤的保护作用。方法神经元分为对照组、氧糖剥夺组、星形胶质细胞外泌体组、缺氧预处理星形胶质细胞外泌体组,除对照组外各组均进行神经元的氧糖剥夺,分别给予等体积PBS、正常细胞外泌体、缺氧预处理细胞外泌体,收集细胞,Western blot方法检测Bax、Caspase 3、Bcl-2表达,流式细胞术检测神经元凋亡率。结果神经元氧糖剥夺后,细胞凋亡率明显上升。与氧糖剥夺组相比,进行外泌体处理能明显降低神经元凋亡率及Bax、Caspase 3的表达、增加Bcl-2表达,其中缺氧预处理星形胶质细胞外泌体组保护作用更强。结论星形胶质细胞外泌体在脊髓神经元氧糖剥夺损伤中具有保护作用,缺氧预处理能增强这种保护作用。Objective To investigate the protective effect of hypoxia preconditioned astrocyte exosomes on spinal neurons in SD rats with oxygen glucose deprivation injury.Methods The neurons were divided into control group,oxygen glucose deprivation group,astrocyte exosomes group and hypoxia precondition astrocyte exosomes group.In addition to the control group,all groups were subjected to oxygen glucose deprivation of neurons,and were given equal volume PBS,normal cellular exosomes,and hypoxic pretreated cellular exosomes,respectively.The expressions of Bax,caspase-3 and Bcl-2 in cells were detected by Western blot,and apoptosis rate of cells was measured by flow cytometry.Results After oxygen and sugar deprivation,the apoptosis rate of neurons was increased significantly.Compared with the oxygen-glucose deprivation group,exosome treatment can significantly reduce apoptosis rate of the neurons,the expressions of Bax and Caspase 3 were decreased,the expression of Bcl-2 was increased,and the hypoxia pretreatment astrocyte exosome group had a stronger protective effect.Conclusion The hypoxia precondition enhanced the protective effect of astrocyte exosomes on the spinal neurons of oxidative glucose deprivation injury.

关 键 词：星形胶质细胞 缺氧预处理 氧糖剥夺 外泌体 凋亡 SD大鼠 

分 类 号：R744[医药卫生—神经病学与精神病学]