基于斑马鱼功效模型的钙果饮补钙效果评价  

Evaluation of Calcium Supplementation Effect of Prunus Humilis Drink Based on Zebrafish Efficacy Model

作　　者：孟瑞丽 李英 明平幸 汪丹 魏贺敏 李桢 MENG Ruii;LI Ying;MING Pingxing;WANG Dan;WEI Hemin;LI Zhen(Shanxi Key Laboratory of Medicinal and Edible Homology Functional Food,Changzhi 047100,China;Shanxi Zhendong Pharmaceutical Co.,Ltd.,Changzhi 047100,China;Shanxi Zhendong Wuhe Medical Care Hall Co.,Ltd.,Changzhi 047100,China)

机构地区：[1]药食同源功能食品山西省重点实验室,山西长治047100 [2]山西振东制药股份有限公司,山西长治047100 [3]山西振东五和医养堂股份有限公司,山西长治047100

出　　处：《食品安全导刊》2023年第26期107-109,共3页China Food Safety Magazine

摘　　要：建立斑马鱼功效模型,验证钙果饮的补钙效果。选择发育健康且生长阶段一致的3 dpf野生型斑马鱼,加入不同浓度(3.125μg·mL^(-1)、6.25μg·mL^(-1)、12.5μg·mL^(-1)、25.0μg·mL^(-1)、50.0μg·mL^(-1)、100.0μg·mL^(-1))钙果饮,同时设置正常对照组,每孔容量为3 mL,确定钙果饮对正常斑马鱼的最大耐受浓度;取3个浓度梯度组(3.125μg·mL^(-1)、6.25μg·mL^(-1)、12.5μg·mL^(-1))、阳性对照组和正常对照组,用钙黄绿素进行染色,计算斑马鱼的椎骨荧光强度。结果表明,钙果饮对正常斑马鱼的最大耐受浓度为12.5μg·mL^(-1);钙果饮的补钙效果随浓度的增加而增加,与空白对照组相比,不同钙果饮浓度处理的斑马鱼骨骼荧光强度增强,说明钙果饮具有显著的补钙效果。Establish a zebrafish efficacy model to verify the calcium supplementation effect of Prunus humilis drink.Select 3 dpf wild-type zebrafish with healthy development and consistent growth stages,and add different concentrations(3.125μg·mL^(-1),6.25μg·mL^(-1),12.5μg·mL^(-1),25.0μg·mL^(-1),50.0μg·mL^(-1),100.0μg·mL^(-1))Prunus humilis drink,while setting up a normal control group with a well volume of 3 mL,determine the maximum tolerable concentration of Prunus humilis drink on normal zebrafish;take 3 concentration gradient groups(3.125μg·mL^(-1),6.25μg·mL^(-1),12.5μg·mL^(-1)),positive control group,and normal control group were stained with calcein to calculate thefluorescence intensity of zebrafish vertebrae.The results indicate that the maximum tolerable concentration of Prunus humilis drink for normal zebrafish is 12.5μg·mL^(-1);the calcium supplementation effect of Prunus humilis drink increases with the increase of concentration.Compared with the blank control group,thefluorescence intensity of zebrafish bones treated with different concentrations of Prunus humilis drink is enhanced,indicating that Prunus humilis drink has a significant calcium supplementation effect.

关 键 词：钙果饮 斑马鱼功效模型 补钙效果 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]