优化溃结方对溃疡性结肠炎气滞血瘀模型大鼠的影响及其作用机制研究  被引量:5

Effect and Mechanism of Optimized Kuijie Decoction in Ulcerative Colitis Rats with TCM Syndrome Differentiation of Qi Stagnation and Blood Stasis

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作  者:张帅 李娜 沈江立 柳越冬[3] 吴宪树 王磊[4] 盛天骄 徐红俊 安胜军 ZHANG Shuai;LI Na;SHEN Jiangli;LIU Yuedong;WU Xianshu;WANG Lei;SHENG Tianjiao;XU Hongjun;AN Shengjun(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Xianyang Central Hospital,Xianyang 712000,China;The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110005,China;Anorectal Department,China-Mongolia Hospital,Hulunbuir 021008,China;Department of Traditional Chinese Medicine,PLA Northern Theater Command General Hospital,Shenyang 110005,China)

机构地区:[1]河北中医药大学,河北省石家庄市050091 [2]陕西省咸阳市中心医院,712000 [3]辽宁中医药大学附属第三医院,辽宁省沈阳市110005 [4]内蒙古自治区呼伦贝尔市中蒙医院肛肠科,021008 [5]解放军北部战区总医院中医科,辽宁省沈阳市110005

出  处:《中国全科医学》2024年第11期1356-1362,共7页Chinese General Practice

基  金:陕西省自然科学基础研究计划项目(2022JM-489);陕西省中医药管理局秦创原中药创新研发项目(2022-QCYZH-015);2023年度河北省社会科学发展研究课题(20230204037)。

摘  要:背景经验方优化溃结方能有效提高溃疡性结肠炎(UC)患者生活质量,促进肠黏膜愈合和临床缓解,但其作用机制尚不完全明确。目的探讨优化溃结方对UC气滞血瘀模型大鼠的影响及其作用机制。方法2023年9—10月,选取SPF级雄性SD大鼠70只并采用随机数字表法将其随机分为正常组、模型组、柳氮磺胺吡啶组、低剂量组、标准剂量组、益气组、活血组,每组10只。采用三硝基苯磺酸(TNBS)/乙醇二次致炎法结合束缚法建立UC气滞血瘀模型。正常组大鼠在造模时使用0.9%氯化钠溶液灌肠,并与其他组大鼠进行同步抓取、固定,在造模成功后给予等体积水灌胃,1次/d,共灌胃14次;模型组大鼠在造模成功后给予等体积水灌胃,1次/d,共灌胃14次;柳氮磺胺吡啶组、低剂量组、标准剂量组、益气组、活血组大鼠分别在造模成功后给予柳氮磺胺吡啶药液0.54 g/kg灌胃、低剂量优化溃结方药液0.837 g/kg、标准剂量优化溃结方药液1.674 g/kg灌胃、黄芪药液1.8 g/kg、红花药液0.9 g/kg,均为1次/d,均灌胃14次。采用高精度透射电镜观察7组大鼠干预后结肠组织超微结构变化,并比较7组大鼠干预后结肠组织趋化因子受体4(CXCR4)、血管内皮生长因子A(VEGFA)、转化生长因子激酶1(TAK1)表达水平。结果低剂量组、标准剂量组大鼠干预后结肠组织超微结构趋于正常。模型组大鼠干预后结肠组织CXCR4、VEGFA、TAK1蛋白灰度比值、mRNA相对表达量高于正常;柳氮磺胺吡啶组、低剂量组、标准剂量组、益气组、活血组大鼠干预后结肠组织CXCR4、VEGFA、TAK1蛋白灰度比值、mRNA相对表达量低于模型组(P<0.05);标准剂量组大鼠干预后结肠组织CXCR4、VEGFA、TAK1蛋白灰度比值低于柳氮磺胺吡啶组,活血组大鼠干预后结肠组织VEGFA、TAK1 mRNA相对表达量低于柳氮磺胺吡啶组(P<0.05)。结论优化溃结方及其益气、活血组分可有效降低UC气滞血�Background Experiential effective prescription optimized Kuijie decoction can effectively improve the quality of life of patients with ulcerative colitis(UC),promote the intestinal mucosal healing and clinical remission,but its mechanism remains unclear now.Objective To investigate the effect and mechanism of optimized Kuijie decoction in UC rats with TCM syndrome differentiation of Qi stagnation and blood stasis.Methods From September to October 2023,70 SPFgrade male SD rats were randomly divided into the blank-control,model,salicylazosulfapyridine,low-dose,standarddose,Qi-enhancing and Blood-activating groups,with 10 rats in each group.UC model with TCM syndrome differentiation of Qi stagnation and blood stasis was established by trinitrobenzenesulfonic acid(TNBS)/ethanol secondary inflammation combined with restraint method.Rats in the blank-control group were enucleated with 0.9%sodium chloride solution at the time of modeling and were grasped and fixed synchronously with the rats in the other groups,and were given an equal volume of water by gavage once per day after the establishing for 14 days;rats in the model group were given an equal volume of water by gavage once per day after the establishing for 14 days;rats in the salicylazosulfapyridine,low-dose,standard-dose,Qi-enhancing and Blood-activating groups were given intragastric administration with salicylazosulfapyridine suspension(0.54 g/kg),low-dose optimized Kuijie decoction(0.837 g/kg),standard-dose optimized Kuijie decoction(1.674 g/kg),Astragalus suspension(1.8 g/kg)and Safflower suspension(0.9 g/kg)once per day after the establishing for 14 days,respectively.High-precision transmission electron microscope was used to observe the ultrastructural changes in the colonic tissues of the seven groups of rats after intervention,the expression levels of CXCR4,VEGFA,and TAK1 were compared among the seven groups of rats after intervention.Results The ultrastructure of colonic tissue of rats in the low-dose and standard-dose groups tended to be normal after

关 键 词:结肠炎 溃疡性 血瘀气滞 大鼠 优化溃结方 药理作用分子作用机制(中药) 

分 类 号:R574.62[医药卫生—消化系统]

 

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