胃癌患者胃黏膜微生物群落结构特征分析  

Analysis of the composition and diversity of gastric microbiome in patients with gastric cancer

作　　者：黄坤[1] 赵晓琳 程建平[1] 吴丽丽[2] HUANG Kun;ZHAO Xiaolin;CHENG Jianping;WU Lili(Department of Gastroenterology,Civil Aviation General Hospital,Civil Aviation Medical College of Peking University,Beijing,100123,China;Department of Gastroenterology, the Second Medical Center, PLA General Hospital)

机构地区：[1]民航总医院(北京大学民航临床医学院)消化内科,北京100123 [2]解放军总医院第二医学中心消化内科

出　　处：《中国中西医结合消化杂志》2024年第1期18-22,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：民航总医院(民航医学中心)院级课题资助项目(No:201920;No:202207)。

摘　　要：目的:通过16S rRNA高通量测序分析胃癌患者的胃黏膜微生物群落结构与组成。方法:选取2020年1月—2022年12月就诊的15例胃癌患者为胃癌组;对照组包括萎缩性胃炎合并肠上皮化生组以及健康对照组2个亚组,每组各15例。收集胃黏膜标本,采用16S rRNA高通量测序技术检测3组受试者的胃黏膜菌群的组成并分析其结构。结果:3组受试者的胃黏膜菌群Alpha多样性差异无统计学意义(P>0.05),但Beta多样性差异有统计学意义(P<0.05)。LEfSe分析发现胃癌组棒状杆菌属、漫游球菌属、链球菌属等丰度差异有统计学意义(P<0.05)。萎缩性胃炎合并肠上皮化生组普雷沃菌属、德沃斯菌属丰度差异有统计学意义(P<0.05)。结论:胃癌患者的胃黏膜微生物群落结构与对照组存在显著差异,棒状杆菌属、漫游球菌属、链球菌属等菌属可能与胃癌的发生、发展相关。Objective:To analyze the composition and diversity of gastric microbiome in patients with gastric cancer by 16S rRNA high-throughput sequencing.Methods:Fifteen gastric cancer patients who attended our hospital from January 2020 to December 2022 were selected as gastric cancer(GC) group,and the control group included 2 subgroups of atrophic gastritis complicated with intestinal metaplasia(IM) and healthy control(HC),with 15 cases in each of the 2 subgroups.Gastric mucosal samples were collected,16S rRNA high-throughput sequencing technology was used to detect the composition and diversity of gastric microbiome in three groups.Results:There was no significant difference in Alpha diversity between the three groups(P>0.05),but there was a significant difference in Beta diversity(P<0.05).LEfSe analysis found that the abundance of Corynebacterium,Vagococcus and Streptococcus in the gastric cancer group was statistically significant(P<0.05).The abundance of Prevotella and Devosia in the group of atrophic gastritis and intestinal metaplasia was statistically significant(P<0.05).Conclusion:There are significant differences between the composition and diversity of gastric microbiome in gastric cancer patients and control,and Corynebacterium,Vagococcus and Streptococcus may be related to the occurrence and development of gastric cancer.

关 键 词：胃癌 微生态 16S rRNA 高通量测序 

分 类 号：R573[医药卫生—消化系统]