CD3D基因与卵巢癌免疫浸润的相关性及其预后价值  

CD3D as a prognostic signature and its correlation with immune infiltration in ovarian cancer

作　　者：张韶华 王艺璇 王赞宏[1] Zhang Shaohua;Wang Yixuan;Wang Zanhong(Department of Obstetrics and Gynaecology,Third Hospital of Shanxi Medical University,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Taiyuan 030032,China)

机构地区：[1]山西医科大学第三医院、山西白求恩医院、山西医学科学院、同济山西医院妇产科,太原030032

出　　处：《中华生物医学工程杂志》2023年第5期481-487,共7页Chinese Journal of Biomedical Engineering

基　　金：山西省科技成果转化引导专项二批项目(201604D132043);山西省基础研究计划(202103021224352);山西省中央引导地方项目(YDZJSX2022B012)。

摘　　要：目的探讨CD3D基因在卵巢癌(OC)组织中的表达,对卵巢癌肿瘤微环境中免疫浸润的影响及其预后价值。方法利用GEPIA数据库分析CD3D基因在包括卵巢癌在内的多种肿瘤中的表达情况;收集2011年1月至2017年12月山西白求恩医院治疗的卵巢癌患者223例及因子宫平滑肌瘤切除全子宫+双侧附件的患者25例,采用免疫组化SP法检测卵巢癌及正常卵巢组织中CD3D基因的蛋白表达,比较CD3D基因表达与卵巢癌患者临床病理特征之间的关系;cBioportal数据库分析CD3D基因的共表达基因;DAVID数据库分析CD3D基因及其共表达基因KEGG功能富集通路;Kaplan Meier plotter数据库分析CD3D基因的预后价值;TIMER2.0数据库分析CD3D基因表达与卵巢癌中主要免疫细胞的免疫浸润关系。结果与正常卵巢组织相比,CD3D蛋白在卵巢癌组织中高表达(P<0.05),且在卵巢癌不同FIGO分期、残留病灶是否达R0间的表达差异均有统计学意义(均P<0.05)。Kaplan-Meier-Plotter预后分析提示CD3D基因高表达的患者具有更高的生存率(P<0.05)。表达基因的富集分析提示,该组基因主要富集于Th1与Th2细胞分化通路、T细胞受体信号通路、Th17细胞分化通路、原发性免疫缺陷通路、PD-L1和PD-1免疫检查点通路、自然杀伤细胞介导的细胞毒性通路等。Timer数据库分析发现CD3D基因与肿瘤微环境中B细胞、CD8+ T细胞、CD4+ T细胞和树突状细胞的浸润程度呈正相关(均P<0.05)。结论 CD3D基因在卵巢癌组织中高表达,其可能通过调整免疫相关通路影响肿瘤微环境中免疫细胞的浸润而改变患者预后。Objective To investigate the expression of CD3D gene in ovarian cancer tissues,its effect on immune infiltration in ovarian cancer tumor microenvironment and its prognostic value.Methods GEPIA database was used to analysis the expression of CD3D gene in various tumors including ovarian cancer;223 cases of ovarian cancer patients and 25 cases of patients with uterine smooth muscle tumor treated in Shanxi Bethune Hospital from January 2011 to December 2017 were collected.25 patients with total uterus+bilateral adnexa were resected,and the protein expression of CD3D gene was detected in ovarian cancer and normal ovarian tissues by immunohistochemistry SP method,comparing the relationship between the expression of CD3D gene and clinicopathological features of patients with ovarian cancer;the Kaplan Meier plotter database was used to analysis the prognostic value of the CD3D gene;the cBioportal database was used to analysis the co-expressed genes of CD3D gene;DAVID database to analysis the KEGG functional enrichment pathway of CD3D co-expressed genes;TIMER2.0 database to analysis the relationship between CD3D gene expression and immune infiltration of major immune cells in ovarian cancer.Results CD3D protein was highly expressed in ovarian cancer tissues,with statistically significant differences compared with normal ovarian tissues(P<0.05),and there was a significant difference in expression between different FIGO staging periods of ovarian cancer,between whether the residual lesions reached R0 or not(P<0.05),and that the high-expression group had a higher survival rate,with a statistically significant difference(P<0.05);Kaplan-Meier-Plotter prognostic analysis also suggested that patients with high CD3D mRNA expression had higher survival rates(P<0.05).The enrichment analysis of the expressed genes suggested that the genes in this group were mainly enriched in Th1 and Th2 cell differentiation pathway,T cell receptor signaling pathway,Th17 cell differentiation pathway,primary immunodeficiency pathway,PD-L1 and PD-1 i

关 键 词：CD3D基因 卵巢癌 免疫浸润 肿瘤微环境 

分 类 号：R737.31[医药卫生—肿瘤]