鼻腔鼻窦鳞状细胞癌中PIK3CA、p-AKT、PTEN的表达及意义  

Expression and significance of PIK3CA,p-AKT and PTEN in sinonasal squamous cell carcinoma

作　　者：赵海清 王佳说 赵一辰 姜菲菲[1] 阎艾慧[1] ZHAO Haiqing;WANG Jiashuo;ZHAO Yichen;JIANG Feifei;YAN Aihui(Department of Otorhinolaryngology,First Affiliated Hospital of China Medical University,Shenyang,Liaoning 110001,China;Research Room of Clinical Epidemiology and Evidence-Based Medicine,First Affiliated Hospital of China Medical University,Shenyang,Liaoning 110001,China)

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉科,沈阳110001 [2]中国医科大学附属第一医院临床流行病学与循证医学研究室,沈阳110001

出　　处：《重庆医学》2024年第2期232-238,共7页Chongqing medicine

摘　　要：目的探讨PIK3CA、磷酸化蛋白激酶B(p-AKT)及10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)在鼻腔鼻窦鳞状细胞癌(SNSCC)中的表达及意义。方法通过UCSC Xena数据库中癌基因组图谱的头颈鳞癌数据集(TCGA-HNSC)分析PIK3CA和PTEN在头颈鳞状细胞癌(HNSCC)中的表达情况。采用免疫组织化学SP法检测43例SNSCC组织和20例正常下鼻甲黏膜组织中PIK3CA、p-AKT及PTEN蛋白表达,分析PIK3CA、p-AKT及PTEN蛋白表达与SNSCC患者的临床病理特征和预后的关系。结果生物信息学分析结果显示,HNSCC组织中PIK3CA mRNA表达高于癌旁组织(P<0.01),而PTEN mRNA表达低于癌旁组织(P<0.05)。免疫组织化学检测结果显示,PIK3CA和p-AKT蛋白在正常下鼻甲黏膜组织中的阳性表达率明显低于SNSCC组织,而PTEN蛋白在SNSCC组织中的阳性表达率明显高于正常下鼻甲黏膜组织,差异有统计学意义(P<0.01)。PIK3CA及p-AKT蛋白表达与临床分期、分化程度、原发部位有关(P<0.05),而与年龄、性别、吸烟、饮酒无关(P>0.05);PTEN蛋白表达与临床分期、分化程度、原发部位、年龄、性别、吸烟、饮酒均无关(P>0.05)。Spearman分析显示,SNSCC组织中PIK3CA和p-AKT蛋白表达呈正相关(r=0.664,P<0.01),PIK3CA与PTEN蛋白表达呈负相关(r=-0.414,P<0.01),p-AKT与PTEN蛋白表达呈负相关(r=-0.453,P<0.01)。Kaplan-Meier分析显示,PIK3CA及p-AKT蛋白阳性表达患者中位生存期短于阴性表达患者(P<0.01),PTEN蛋白阳性表达患者中位生存期与阴性表达患者比较差异无统计学意义(P>0.05)。结论PIK3CA和p-AKT过表达伴PTEN表达缺失参与SNSCC的发生、发展,且PIK3CA和p-AKT表达与患者不良预后相关。Objective To investigate the expression of PIK3CA,phosphorylated protein kinase B(p-AKT)and phosphatase and tensin homologue deleted on chromosome 10(PTEN)in sinonasal squamous cell carcinoma(SNSCC).Methods The expressions of PIK3CA and PTEN in head and neck squamous cell carcinoma(HNSCC)were analyzed through the data set of HNSCC in the cancer genome map of UCSC Xena database.The immunohistochemical SP method was used to measure the expression of PIK3CA,p-AKT and PTEN in 43 cases of SNSCC tissues,20 cases of normal inferior concha tissues.The relationship between the expressions of PIK3CA,p-AKT and PTEN protein with the clinicopathological features and prognosis of the patients with SNSCC was analyzed.Results The results of bioinformatic analysis showed that PIK3CA mRNA expression in HNSCC tissues was higher than that in paracancerous tissues(P<0.01),while the PTEN mRNA expression was lower than that in paracancerous tissues(P<0.05).The immunohistochemical detection results showed that the positive expressions rates of PIK3CA and p-AKT proteins in normal nasal mucosa tissues were significantly lower than those in SNSCC tissues,while the positive expression rate of PTEN protein in SNSCC tissues was significantly higher than that in normal inferior nasal concha mucosa tissues,and the differences were statistically significant(P<0.01).The expressions of PIK3CA and p-AKT protein were related to the clinical stage,differentiation degree and primary site(P<0.05),but were not related to age,gender,smoking and drinking(P>0.05);the PTEN protein expression was not related with the clinical stage,differentiation degree,primary site,age,smoking and drinking(P>0.05).The Spearman analysis showed that the expression of PIK3CA in SNSCC tissues was positively correlated with p-AKT protein expression(r=0.664,P<0.01),and PIK3CA was negatively correlated with PTEN protein(r=-0.414,P<0.01).The expression of p-AKT was negatively correlated with PTEN protein(r=-0.453,P<0.01).The Kaplan-Meier analysis showed that the median survival ti

关 键 词：PIK3CA PTEN 磷酸化蛋白激酶B 鼻腔鼻窦 鳞状细胞癌 

分 类 号：R739.62[医药卫生—肿瘤]