安徽新安江水牛mtDNA D-Loop区遗传多样性与系统进化研究  

Genetic Variation and Phyletic Evolution of Xin′anjiang Buffalo Based on Mitochondrial DNA D-Loop

作　　者：赵拴平[1] 金海 刘峻 李永胜 金磊 李倩 徐磊[1] 贾玉堂[1] ZHAO Shuanping;JIN Hai;LIU Jun;LI Yongsheng;JIN Lei;LI Qian;XU Lei;JIA Yutang(Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering,Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Xiuning County Husbandry and Veterinary Promotion Station,Xiuning 245000,China;Huangshan Husbandry and Veterinary Promotion Station,Huangshan 245700,China)

机构地区：[1]安徽省农业科学院畜牧兽医研究所,畜禽产品安全工程安徽省重点实验室,合肥230031 [2]休宁县畜牧兽医技术推广站,休宁245000 [3]黄山市畜牧兽医推广中心,黄山245700

出　　处：《中国草食动物科学》2024年第1期1-7,共7页China Herbivore Science

基　　金：安徽省肉牛良种联合攻关项目([2021]1146);安徽省科技重大专项(S202003b06020001)。

摘　　要：试验旨在分析安徽省黄山市新安江流域上游地区新安江水牛群体的分子遗传特性,探究其母系起源与遗传多样性。利用PCR扩增和测序技术测定28头新安江水牛的mtDNA D-Loop序列,下载GenBank数据库中24个中国水牛群体的693条D-Loop序列,利用生物信息学分析其遗传多样性,构建Neighbor-joining系统发生树和Media-joining网络,探索不同水牛群体的遗传距离。结果显示,28头新安江水牛的mtDNA D-Loop序列共有117个变异位点,构成25种单倍型,其核苷酸多样性为0.02602±0.00303,单倍型多样性为0.989±0.014。新安江水牛群体的变异性水平与中国其他水牛群体接近。N-J系统进化树显示,新安江水牛25个单倍型分为A、B两个支系,具有A支系和B支系2个母系来源,其中A支系占据主导地位。Media-joining进化网络显示,中国水牛主要为沼泽型水牛,分为沼泽型水牛A支系和B支系,B支系又分为b1亚支系和b2亚支系。综上,新安江水牛群体变异水平与中国其他地方水牛群体接近,群体遗传多样性丰富;且新安江水牛属于沼泽型水牛,具有2个线粒体母系来源,与我国其他地方水牛群体具有一定的遗传距离。The objectives of this study were to investigate molecular genetic characteristics of Xin′anjiang buffalo in the upper reaches of Xin′an River basin of Huangshan city from Anhui province,and explore the maternal origin and genetic diversity.The mtDNA D-Loop sequences of 28 Xin′anjiang buffalo were sampled and determined by PCR amplification and sequencing.693 D-Loop sequences of 24 Chinese buffalo were downloaded from GenBank database.Genetic bioinformatics were used to analyze genetic diversity,construct Neighbor-joining tree and Media-joining network,and explore the distance between populations.The results showed that 117 nucleotide polymorphic sites and 25 haplotypes were identified in Xin′anjiang buffalo population,the estimated overall nucleotide diversity and haplotype diversity were 0.02602±0.00303 and 0.989±0.014,respectively.Genetic variation of Xin′anjiang buffalo was similar to other buffalo populations.The Neighbor-joining tree indicated that the 25 haplotypes of Xin′anjiang buffalo were divided into A and B lines,with 2 maternal sources,of which A was dominant.The Media-joining evolutionary network showed that Chinese buffalo were mainly swamp buffalo,including A and B lines,and the B line could be further divided into sub-lineages of b1 and b2.In summary,there was rich mitochondrial genetic diversity in Xin′anjiang buffalo population,and it was similar to other buffaloes in China;the Xin′anjiang buffalo was a swamp buffalo with two mitochondrial maternal sources,which had a certain genetic distance from other buffalo populations in China.

关 键 词：水牛 线粒体DNA 遗传多样性 单倍型 

分 类 号：S823.2[农业科学—畜牧学]