基于定量构效关系制备火麻仁蛋白ACE抑制肽  

Preparation of ACE Inhibitory Peptide of Hemp Seed ProteinBased on Quantitative Structure-Activity Relationship

作　　者：刘梦琪 刘畅[1,2] 刘博浩 阴雨奇 张瀚林 张英华[1,2] 董艳[3] Liu Mengqi;Liu Chang;Liu Bohao;Yin Yuqi;Zhang Hanlin;Zhang Yinghua;Dong Yan(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030;College of Food Science,Northeast Agricultural University,Harbin 150030;Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang)

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学食品学院,哈尔滨150030 [3]黑龙江省科学院大庆分院,黑龙江大庆163319

出　　处：《中国食品学报》2023年第12期143-152,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省现代农业产业技术协同创新推广体系麻类(工业)(SCXTI(MLGY)2019)。

摘　　要：高血压是引发心血管疾病的主要因素,经常服用降压的药物会产生副作用,因而安全、高效的食源性降压肽成为关注点。本研究建立了血管紧张素转换酶(ACE)抑制肽的定量构效关系(QSAR)模型,确定ACE抑制肽的结构与其活性间的关系。将模型结果辅以计算机虚拟酶切精确掌握蛋白水解位点,筛选合适的蛋白酶水解火麻仁蛋白定向制备ACE抑制肽,并测定最终水解物的抑制效果。结果表明:基于二肽建立的SVM-AAindex模型具有最佳的预测性能(R~2为0.81,RMSE为0.53),且当二肽C端氨基酸为疏水性和芳香族氨基酸时具有较强的ACE抑制效果。蛋白质组分分析发现火麻仁蛋白含有34.60%的疏水性氨基酸和芳香族氨基酸是制备ACE抑制肽的良好来源。选择虚拟酶切产生较多短肽和生物活性肽的碱性蛋白酶和枯草杆菌蛋白酶对火麻仁蛋白进行定向水解,获得的最终水解液的ACE抑制IC50值分别为1.89和2.30 mg/mL。本研究建立了一种高效精准的制备火麻仁蛋白ACE抑制肽的方法,在降血压药物上具有很好的应用前景。High blood pressure is a major risk factor for cardiovascular disease,and frequent use of blood pressure medications can have side effects.Therefore,safe and efficient food-borne antihypertensive peptides have become the focus of attention.In this study,a quantitative structure-activity relationship(QSAR)model of angiotensin-converting enzyme(ACE)inhibitory peptide was established to determine the relationship between the structure and activity of ACE inhibitory peptide.The model results were combined with computer virtual enzyme digestion to determine proteolytic sites accurately.Suitable proteases were selected to hydrolyze hemp seed protein to prepare ACE inhibitory peptide,and the inhibitory effect of the final hydrolysate was determined.The results showed that the SVM-AAindex model based on dipeptide had the best predictive performance(R20.81,RMSE 0.53),and had a strong ACE inhibition effect when the C-terminal amino acids of dipeptide were hydrophobic and aromatic amino acids.The analysis of protein components showed that hemp seed protein contains 34.60%hydrophobic amino acids and aromatic amino acids,which is a good source for preparing ACE inhibitory peptides.The alkaline and subtilisin,which produced more short peptides and bioactive peptides by virtual enzyme digestion,were selected for directed hydrolysis of hemp seed protein.The ACE inhibition values of the final hydrolysates were 1.89 and 2.30 mg/mL,respectively.In this study,an efficient and accurate method for preparing ACE inhibitory peptide from hemp seed protein was established,which has a good application prospect in antihypertensive drugs.

关 键 词：火麻仁蛋白 ACE抑制肽 QSAR模型 定向制备 

分 类 号：TQ464.7[化学工程—制药化工]