金刚刺地龙汤对弗氏佐剂诱导类风湿关节炎大鼠的影响  

Effect of Smilacis Chinae Rhizoma Lumbricus decoction on freund’s adjuvant Induced rheumatoid arthritis induced

作　　者：张浩敏 赵培源[1] 张信昕 杜汝番 阴蔓茹 徐晨雨 胡飞雪 宗斌 ZHANG Haomin;ZHAO Peiyuan;ZHANG Xinxin;DU Rufan;YIN Manru;XU Chenyu;HU Feixue;ZONG Bin(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046

出　　处：《现代中西医结合杂志》2023年第22期3121-3125,3177,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河南省本科高校省级大学生创新创业训练计划项目(S202110471031)。

摘　　要：目的观察金刚刺地龙汤对完全弗氏佐剂诱导类风湿关节炎大鼠的抗炎作用及对踝关节组织的影响。方法将24只健康雌性SD大鼠随机分为空白组、模型组、双氯芬酸钠组、金刚刺地龙汤组,每组6只。除空白组外,其余组大鼠采用左后足跖部皮内注射0.1 mL完全弗式佐剂方法制备类风湿关节炎模型。实验第14天开始,双氯芬酸钠组给予双氯芬酸钠溶液9 mg/(kg·d)灌胃,金刚刺地龙汤组给予金刚刺地龙汤药液10.62 g/(kg·d)灌胃,空白组和模型组给予等量生理盐水灌胃,均1次/d,连续14 d。实验期间观察各组大鼠行为学表现;评估各组大鼠造模前及实验第1,3,7,10,14,16,19,22,29天左后肢足跖炎症程度,测量足踝体积、足踝围;灌胃结束后处死大鼠,计算脾脏指数,HE染色观察踝关节组织病理学形态,使用ELISA法测定血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)水平。结果模型组大鼠精神状态萎靡,反应迟钝;各时间点足跖炎症程度评分、足踝体积、足踝围及脾脏指数和血清中IL-6、IL-1β、TNF-α水平均明显高于空白组(P均<0.05);关节软骨组织严重破坏,大量炎细胞浸润,毛细血管增生。与模型组比较,双氯芬酸钠组、金刚刺地龙汤组大鼠精神状态与活动程度均明显改善;实验第22天和实验第29天左后肢足跖炎症程度评分、实验第16,19,22,29天足容积和足踝围均明显低于同期模型组(P均<0.05),且金刚刺地龙汤组大鼠实验第22天足容积明显低于同期双氯芬酸钠组(P<0.05);脾脏指数均明显增高(P均<0.05),血清中IL-6、IL-1β、TNF-α水平均明显降低(P均<0.05);骨侵蚀及炎细胞浸润、毛细血管增生均明显减轻。结论金刚刺地龙汤可明显减轻完全弗氏佐剂诱导类风湿关节炎大鼠足趾肿胀和关节组织破坏,机制可能与下调炎症因子水平、减轻炎症反应有关。Objective It is to observe the anti-inflammatory effect of Smilacis Chinae Rhizoma Lumbricus decoction(SCRLD)on rheumatoid arthritis(RA)induced by complete Freund’s adjuvant in rats and its histopathological effect on inflamed ankle.Methods Twenty-four healthy female SD rats were randomly divided into blank control group,model group,diclofenac sodium group and SCRLD group,with 6 rats in each group.All the rats of every group except for the blank control group were given intradermal injection of 0.1 mL complete Freund’s adjuvant in the left hind paw to establish arthritis models.On the 14 th day of modeling,the rats in the diclofenac sodium group were intragastrically administered with 9 mg/(kg·d)diclofenac sodium solution,and the rats in the SCRLD group were intragastrically administered with 10.62 g/(kg·d)SCRLD,the rats in the blank control group and the model group were given the same amount of normal saline by gavage,all once daily,continuously treated for 14 days.During the experiment,the behavioral performance of rats in each group was observed,the degrees of toe inflammation in the left hind limb of rats were assessed before modeling and on days 1,3,7,10,14,16,19,22,and 29 of the experiment in all groups,and the ankle volume,ankle circumference of rats in each group were measured.The rats were executed at the end of gavage,their splenic index was calculated,the histopathological morphology of the ankle joints was observed by HE staining,and the serum levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β)were determined by ELISA.Results The rats in the model group were depressed and unresponsive,their toe inflammation degree scores,ankle volume,ankle circumference and splenic index and serum levels of IL-6,IL-1βand TNF-αwere all significantly higher than those of the blank group at all time points(all P<0.05),and their articular cartilaginous tissues were severely damaged,with a large number of inflammatory cells infiltrated,and capillary hyperplasia.Compared with

关 键 词：金刚刺 地龙 类风湿关节炎 佐剂性关节炎 大鼠 

分 类 号：R-332[医药卫生]