生物样本中短链脂肪酸含量测定的实验教学改进研究  

Study on Experimental Improvement of the Short Chain Fatty Acid Determination in Biological Samples

作　　者：康安[1] 邓海山[1] Kang An;Deng Haishan(School of Pharmacy,Nanjing University of Chinese Medicine,Jiangsu,210000)

机构地区：[1]南京中医药大学药学院,江苏210000

出　　处：《当代化工研究》2023年第24期150-152,共3页Modern Chemical Research

基　　金：2022年度校本科教育教学改革研究课题“中药学卓越人才培养模式创新研究”(项目编号:NZYJG2022002);全国中医药高等教育“十四五”规划2023年度教育科研课题“新时代中药学拔尖人才培养研究”(项目编号:ZD-23-01)。

摘　　要：采用程序升温气相色谱法测定五种短链脂肪酸是我校开设的仪器分析实验课程中的一个综合性设计性实验,但在教学过程中仍存在一些不足。我们针对上述实验的不足,进行如下改进,包括:(1)分析对象明确为生物样本中的短链脂肪酸;(2)采用化学衍生化法获得短链脂肪酸的衍生化产物;(3)采用液相色谱-质谱对产物进行检测,加深学生对质谱仪相关理论知识的理解;(4)加入开放性实验元素。采用此改进实验开展教学,可提高学生综合运用所学知识解决实际问题的能力,也有利于培养学生的创新精神。Determining five Short Chain Fatty Acids(SCFAs)by gas chromatography with temperature programming is a comprehensive designed experiment in the course of instrumental analysis experiment in our school.Yet,there are some shortcomings during the undergraduate teaching process.In view of the shortcomings above,we come up with the following improved scheme for determining the SCFAs in biological samples.These include:(1)The objective of the improved experiment is to determine the SCFAs concentration in real biological samples;(2)The derivative products of SCFAs are obtained by chemical derivation;(3)By using LC-MS to detect the derivation products,further deepening the students’understanding of relevant theoretical knowledge of MS;(4)Add open experimental elements.Using the improved experiment,we can enhance the student’s ability to comprehensively apply knowledge to solve practical problems while teaching,which is also helpful to cultivate their spirit of learning.

关 键 词：综合设计性实验 实验改进 仪器分析实验 

分 类 号：G642[文化科学—高等教育学]