一种注射式软骨移植体的构建及应用探究  

Construction and application of an injectable cartilage graft

作　　者：马超群 李海强 吴炜 MA Chaoqun;LI Haiqiang;WU Wei(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,The Third Hospital Affiliated of Air Force Military Medical University,China,Xi'an 710032)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学第三附属医院口腔颌面外科,西安710032

出　　处：《实用口腔医学杂志》2024年第1期20-25,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金面上项目(编号:81771040)。

摘　　要：目的:探索一套流程实现软骨的高效颗粒化处理,与富血小板凝胶(PRG)构建注射式的软骨移植体,并利用裸鼠模型评估其效果。方法:取成年雄兔的肋软骨,切割为碎块,置入研钵依次进行液氮冷冻和30~60 s研磨获得颗粒软骨;从耳中动脉采集血液,二次离心法制备PRG。动物实验:对照组(n=5)为颗粒软骨,实验组(n=5)以颗粒软骨和PRG混合,分别注射于裸鼠皮下,8周后进行取材,观察大体形态并进行组织学观察。以实验组构建裸鼠鼻背增高功能模型(n=5)。结果:冷冻研磨处理后颗粒直径分布在10~500μm范围,可实现注射;体外培养证实颗粒软骨的细胞存活率能够达到80%。动物实验取材显示,对照组形态保持较差,存在明显颗粒感;实验组形态保持良好,表面光滑可见明显毛细血管;实验组在裸鼠鼻背增高模型实现显著的增高效果。结论:冷冻研磨技术能实现软骨的高效颗粒化处理,与PRG构建移植体适用颌面部软骨移植修复。Objective:To explore a set of procedures for the effictive granulation of cartilage,and to construct injectable cartilage graft with platelet-rich gel(PRG),and to evaluate its effects in nude mice models.Methods:The costal cartilage of adult male rabbits was cut into pieces and placed in a mortar for 30-60 s freezing in liquid nitrogen and grinding for a short time to obtain granular cartilage.Blood was collected from the middle ear artery and PRG was prepared by double centrifugation.Granular cartilage and the mixture of granular cartilage and PRG were injected subcutaneously in nude mice of control group(n=5)and experimental group(n=5)respectively.8 weeks after injection,the gross morphology observation and histological examination were pertormed.The nasal dorsum augmentation model of nude mice was established in the experimental group.Results:The cartilage particles were in the size of 10-500μm in diameter after frozen grinding,and injection could be realized.In vitro culture showed that the cell survival rate of granular cartilage reached to 80%.In vivo test showed that the sample morphology of the control group was insignificant,and the surface was grainy.That of the experimental group was well maintained,and the surface was smooth with obvious capillaries.The samples of experimental group achieved significant augmentation in the nude mouse model of nasal dorsum augmentation.Conclusion:Freeze-milling technology can achieve efficient granulation of cartilage,and the graft constructed with PRG gel is suitable for maxillofacial cartilage transplantation repair.

关 键 词：注射式 软骨移植 鼻背增高 

分 类 号：R782.2[医药卫生—口腔医学]