结直肠癌组织miR-137-3p、miR-410-3p表达水平与上皮间充质转化和预后的关系分析  

Analysis of the Relationship between the Expression Levels of miR-137-3p and miR-410-3p in Colorectal Cancer Tissue and Epithelial Mesenchymal Transition and Prognosis

作　　者：季从飞[1] 倪婷婷 王建红[1] 于洋 苏小琴[1] JI Cong-fei;NI Ting-ting;WANG Jian-hong;YU Yang;SU Xiao-qin(Department of Medical Oncology,Nantong University Affiliated Cancer Hospital North Hospital,Nantong,Jiangsu,226006,China)

机构地区：[1]南通大学附属肿瘤医院北院肿瘤内科,江苏南通226006

出　　处：《现代生物医学进展》2023年第23期4550-4554,共5页Progress in Modern Biomedicine

基　　金：江苏省自然科学基金项目(BK20191208)。

摘　　要：目的:探讨结直肠癌(CRC)组织微小RNA-137-3p(miR-137-3p)、微小RNA-410-3p(miR-410-3p)的表达与上皮间充质转化(EMT)和预后的关系。方法:选取2017年2月至2019年2月本院收治的115例接受CRC根治术治疗的患者,采用实时荧光定量聚合酶链式反应(qRT-PCR)检测癌组织及癌旁组织中miR-137-3p、miR-410-3p表达、EMT标志物E-钙粘蛋白(E-cadherin)、波形蛋白(Vimentin)的mRNA表达并进行相关性分析,分析癌组织中miR-137-3p、miR-410-3p表达与CRC临床病理特征的关系。术后随访3年,采用Kaplan-Meier法分析miR-137-3p、miR-410-3p高表达和低表达患者的预后情况。结果:癌组织中miR-137-3p表达及E-cadherin mRNA表达水平低于癌旁组织,miR-410-3p表达及Vimentin mRNA表达水平高于癌旁组织(P<0.05)。癌组织中miR-137-3p表达与E-cadherin mRNA表达水平、miR-410-3p表达与Vimentin mRNA表达水平分别呈正相关(P<0.05),癌组织中miR-137-3p表达与Vimentin mRNA表达水平、miR-410-3p表达与E-cadherin mRNA表达水平分别呈负相关(P<0.05)。miR-137-3p、miR-410-3p表达与TNM分期、肿瘤分化程度、淋巴结转移有关(P<0.05)。Kaplan-Meier生存曲线分析显示,miR-137-3p低表达、miR-410-3p高表达患者3年累积生存率下降(P<0.05)。结论:CRC组织中miR-137-3p表达下调、miR-410-3p表达上调,miR-137-3p、miR-410-3p表达水平与EMT密切相关,且miR-137-3p高表达、miR-410-3p低表达患者的预后更好。Objective:To investigate the relationship between the expression of microRNA-137-3p(miR-137-3p)and microRNA-410-3p(miR-410-3p)in colorectal cancer(CRC)tissue and epithelial mesenchymal transition(EMT)and prognosis.Methods:115 patients who received CRC radical surgery in our hospital from February 2017 to February 2019 were selected.Real time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-137-3p,miR-410-3p,and the mRNA expression of EMT markers E-cadherin,Vimentin in cancer tissues and adjacent tissues.The relationship between miR-410-3p expression and clinical pathological characteristics of CRC were analyzed.Follow up for 3 years after surgery,Kaplan Meier method was used to analyze the prognosis of patients with high and low expression of miR-137-3p and miR-410-3p.Results:The expression levels of miR-137-3p and E-Cadherin mRNA in cancer tissues were lower than those in adjacent tissues,while the expression levels of miR-410-3p and Vimentin mRNA were higher than those in adjacent tissues(P<0.05).The expression of miR-137-3p in cancer tissue was positively correlated with the expression level of E-cadherin mRNA,and the expression level of miR-410-3p was positively correlated with the expression level of Vimentin mRNA,respectively(P<0.05).The expression level of miR-137-3p in cancer tissue was negatively correlated with the expression level of Vimentin mRNA,and the expression level of miR-410-3p was negatively correlated with the expression level of E-cadherin mRNA,respectively(P<0.05).The expression of miR-137-3p and miR-410-3p was related to TNM staging,tumor differentiation,and lymph node metastasis(P<0.05).Kaplan Meier survival curve analysis showed that patients with low expression of miR-137-3p and high expression of miR-410-3p had a decreased 3-year cumulative survival rate(P<0.05).Conclusion:The expression of miR-137-3p is down regulated and miR-410-3p is up regulated in CRC tissues.The expression levels of miR-137-3p and miR-410-3p are closely related

关 键 词：结直肠癌 微小RNA-137-3p 微小RNA-410-3p 临床病理特征 上皮间充质转化 预后 

分 类 号：R735.3[医药卫生—肿瘤]