^(18)F-FDG-PET/CT糖代谢参数与B7-H3、CD8^(+)T细胞在结直肠癌中的相关性研究  

Correlation Between ^(18)F-FDG-PET/CT Glucose Metabolism Parameters and B7-H3,CD8^(+)T Cells in Colorectal Cancer

作　　者：龚淞楠 吴润达[2] 韩野[2] 栾富娟[1] 桑士标[3] 郭凌川[4] 陈卫昌[1] Songnan;WU Runda;HAN Ye;LUAN Fujuan;SANG Shibiao;GUO Lingchuan;CHEN Weichang(Department of Gastroenterology,the First Affiliated Hospital of Soochow University,Suzhou,Jiangsu Province215008;Department of General Surgery,the First Affiliated Hospital of Soochow University,Suzhou,Jiangsu Province215008;Department of Nuclear Medicine,the First Affiliated Hospital of Soochow University,Suzhou,Jiangsu Province215008;Department of Pathology,the First Affiliated Hospital of Soochow University,Suzhou,Jiangsu Province215008)

机构地区：[1]苏州大学附属第一医院消化内科,215008 [2]苏州大学附属第一医院普外科,215008 [3]苏州大学附属第一医院核医学科,215008 [4]苏州大学附属第一医院病理科,215008

出　　处：《胃肠病学》2023年第4期200-207,共8页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金(82073156)。

摘　　要：背景:糖酵解功能与结直肠癌的增殖、转移、耐药性等具有明显相关性,对如何量化评估糖酵解水平还缺乏相应指标。目的:探讨术前^(18)F-FDG-PET/CT糖代谢参数与结直肠癌患者临床病理特征、B7-H3、CD8^(+)T细胞表达的相关性。方法:收集2021年4月—2022年7月苏州大学附属第一医院确诊的40例结直肠腺癌患者,所有患者在术前2周内行全身^(18)F-FDG-PET/CT检查,获取最大标准化摄取值(SUVmax)、平均标准化摄取值(SUVmean)、肿瘤代谢体积(MTV)、病灶糖酵解总量(TLG)。采用免疫组化染色检测B7-H3、CD8^(+)T细胞表达,并分析其与临床病理特征、^(18)F-FDG-PET/CT糖代谢参数的相关性。结果:MTV、TLG与结直肠癌患者的T分期、淋巴结转移相关(P<0.05),而SUVmax、SUVmean、MTV、TLG与其他临床病理特征无关(P>0.05)。与癌旁组织相比,结直肠癌组织中B7-H3表达显著升高(P<0.001),CD8^(+)T细胞表达显著降低(P<0.001)。结直肠癌组织中B7-H3表达与CD8^(+)T细胞表达无关(P>0.05);B7-H3表达与T分期、淋巴结转移相关(P<0.05);CD8^(+)T细胞表达与肿瘤大小、分化程度相关(P<0.05)。结直肠癌组织中B7-H3表达与SUVmax、SUVmean、MTV和TLG相关(P<0.05),CD8^(+)T细胞与MTV、TLG相关(P<0.01)。结论:^(18)F-FDG-PET/CT糖代谢参数中的MTV和TLG能更有效地反映结直肠癌病理特征和转移情况,可能具有更高的评估价值。在结直肠癌组织中,B7-H3明显高表达、CD8^(+)T细胞明显低表达,两者与PET/CT糖代谢参数均存在相关性。联合^(18)F-FDG-PET/CT和B7-H3、CD8^(+)T细胞的表达情况可能为结直肠癌临床判断分期、转移以及评估糖酵解水平等提供更为可靠的参考价值。Background:Glycolytic function is obviously related to the proliferation,metastasis and drug resistance of colorectal cancer,and there is still a lacking of corresponding indicators for quantitatively evaluating the level of glycolysis.Aims:To investigate the correlation between ^(18)F-FDG-PET/CT glucose metabolism parameters and clinicopathological features,as well as the expressions of B7-H3 and CD8^(+)T cells in colorectal cancer patients.Methods:Forty patients with colorectal adenocarcinoma from April 2021 to July 2022 at the First Affiliated Hospital of Soochow University were recruited.All patients underwent whole-body ^(18)F-FDG-PET/CT examination within 2 weeks before surgery,the maximum standardized uptake value(SUVmax),average standardized uptake value(SUVmean),metabolic tumor volume(MTV),and total lesion glycolysis(TLG)were measured.Expressions of B7-H3 and CD8^(+)T cells were determined by immunohistochemistry,and their correlation with clinicopathological features,^(18)F-FDG-PET/CT glucose metabolism parameters were analyzed.Results:MTV,TLG were correlated with T staging and lymph node metastasis in colorectal cancer patients(P<0.05),however,SUVmax,SUVmean,MTV,TLG were not correlated with other clinicopathological features(P>0.05).Compared with paracancerous tissue,expression of B7-H3 in colorectal cancer tissue was significantly increased(P<0.001),while expression of CD8^(+)T cells was significantly decreased(P<0.001).Expression of B7-H3 was not correlated with the expression of CD8^(+)T cells in colorectal cancer tissue(P>0.05).Expression of B7-H3 was correlated with T staging,lymph node metastasis(P<0.05).Expression of CD8^(+)T cells in colorectal cancer tissue was correlated with tumor size and differentiation degree(P<0.05).There was a significant correlation between the expression of B7-H3 in colorectal cancer tissue and SUVmax,SUVmean,MTV,and TLG(P<0.05),while expression of CD8^(+)T cells was correlated with MTV,TLG(P<0.01).Conclusions:MTV and TLG in ^(18)F-FDG-PET/CT glucose metabolism parame

关 键 词：结直肠肿瘤 ^(18)F-FDG-PET/CT B7-H3 CD8^(+)T细胞 糖酵解 

分 类 号：R735.34[医药卫生—肿瘤] R730.44[医药卫生—临床医学]