铜绿假单胞菌适配体的获得及应用  

Aptamer of Pseudomonas aeruginosa:Acquiring and Application

作　　者：刘星雨 李洁 朱龙佼 李相阳 许文涛[3] LIU Xing-yu;LI Jie;ZHU Long-jiao;LI Xiang-yang;XU Wen-tao(College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083;Faculty of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206;Department of Nutrition and Health,China Agricultural University,Beijing 100083)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京农学院食品科学与工程学院,北京102206 [3]中国农业大学营养与健康系,北京100083

出　　处：《生物技术通报》2024年第1期186-193,共8页Biotechnology Bulletin

基　　金：北京市设施蔬菜创新团队项目(BAIC01);国家自然科学基金-国家重大科研仪器研制项目(21927812);青年人才托举工程(BYESS2022133);北京市科技计划课题(Z221100007122004);中国农业大学2115人才工程(00109012)。

摘　　要：铜绿假单胞菌是一种专性需氧的革兰氏阴性菌,是引起人类机会性感染的最主要的微生物,对人类造成多种健康威胁。因此,快速、灵敏、特异的检测对预防及治疗铜绿假单胞菌感染十分关键。目前常规的检测方法仍存在耗时久、成本高、操作难度大等问题,因此需要更加高效经济的检测方法。适配体是一种从随机文库中筛选出来的寡核苷酸,能够形成多种复杂的二级结构,近年来,由于其易于合成和修饰,并且具有高亲和力和强特异性而受到了广泛的关注,也涌现出了大量基于适配体的铜绿假单胞菌检测和防治的研究。本文总结了目前铜绿假单胞菌适配体的筛选和裁剪方面的研究进展,并从铜绿假单胞菌的检测和防治两个方面对其适配体的应用进行了介绍,以期为铜绿假单胞菌适配体的开发和应用提供参考。Pseudomonas aeruginosa is an obligate aerobic Gram-negative bacterium,which is the most important microorganism causing opportunistic infections in humans and poses a variety of health threats to humans.Thus,rapid,sensitive and specific detection is crucial for the prevention and treatment of P.aeruginosa infection.At present,there are problems for conventional detection methods,such as time-consuming,high cost and difficult operation,thus more efficient and economical detection methods are needed.Aptamers are oligonucleotides screened from random libraries that can form a variety of complex secondary structures.In recent years,aptamers have attracted widespread attention due to their ease of synthesis and modification,high affinity and strong specificity,and numerous studies of detection,the prevention and treatment of P.aeruginosa based on aptamers have emerged.In this review,the current progresses in screening and tailoring of P.aeruginosa aptamers were summarized,and the application of its aptamers were introduced from two aspects of detection and control of P.aeruginosa,aiming to provide a reference for the development and application of P.aeruginosa aptamer.

关 键 词：铜绿假单胞菌 适配体 筛选 裁剪 检测 抑菌 治疗 

分 类 号：R378.991[医药卫生—病原生物学]